| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MORC4 |
| Uniprot No | Q8TE76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-486aa |
| 氨基酸序列 | GIRLSTMSPRYLQSNSSSHTRPFSAIAELLDNAVDPDVSARTVFIDVEEVKNKSCLTFTDDGCGMTPHKLHRMLSFGFTDKVIKKSQCPIGVFGNGFKSGSMRLGKDALVFTKNGGTLTVGLLSQTYLECVQAQAVIVPIVPFNQQNKKMIITEDSLPSLEAILNYSIFNRENDLLAQFDAIPGKKGTRVLIWNIRRNKNGKSELDFDTDQYDILVSDFDTEEKMTGGVTSELPETEYSLRAFCGILYMKPRMKIFLRQKKVTTQMIAKSLANVEYDTYKPTFTNKQVRITFGFSCKNSNQFGIMMYHNNRLIKSFEKVGCQVKPTRGEGVGVIGVIECNFLKPAYNKQDFEYTKEYRLTINALAQKLNAYWKEKTSQDNFETSTVARPIPKVPDQTWVQCDECLKWRKLPGKIDPSMLPARWFCYYNSHPKYRRCSVPEEQELTDEDLCLSKAKKQE |
| 预测分子量 | 57.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MORC4重组蛋白的参考文献示例(注：以下文献为虚构示例，仅作格式参考，建议通过学术数据库查询真实文献)：
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1. **标题**：*"Recombinant MORC4 Protein Expression and Its ATPase Activity in Cancer Cell Lines"*
**作者**：Zhang Y, et al.
**摘要**：本研究成功构建了MORC4重组蛋白的原核表达系统，纯化后检测到其具有ATP酶活性，并发现该活性在乳腺癌细胞系中显著上调，提示MORC4可能通过能量依赖机制参与肿瘤发生。
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2. **标题**：*"Structural Insights into the Chromatin Remodeling Function of MORC4"*
**作者**：Smith JL, et al.
**摘要**：通过X射线晶体学解析了重组人源MORC4蛋白的N端结构域，揭示了其与DNA结合的分子基础，并证明该结构域对染色质重塑活性至关重要。
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3. **标题**：*"MORC4 Interacts with p53 via Recombinant Protein Pull-down Assays"*
**作者**：Li H, et al.
**摘要**：利用重组MORC4蛋白进行体外结合实验，发现其与肿瘤抑制因子p53直接相互作用，可能通过调控p53稳定性影响结直肠癌的化疗耐药性。
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4. **标题**：*"Recombinant MORC4 Mutants Reveal Key Residues for Epigenetic Silencing"*
**作者**：Brown KA, et al.
**摘要**：通过构建MORC4重组蛋白的系列点突变体，鉴定了其CW结构域中参与基因沉默和组蛋白修饰的关键氨基酸位点，为靶向表观遗传治疗提供依据。
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建议通过**PubMed**或**Web of Science**检索真实文献，关键词：`MORC4 recombinant protein`、`MORC4 structure/function`。
The MORC4 (Microrchidia family CW-type zinc finger protein 4) recombinant protein is derived from the MORC4 gene, a member of the evolutionarily conserved MORC family involved in chromatin remodeling, transcriptional regulation, and epigenetic silencing. MORC proteins are characterized by a conserved GHKL ATPase domain and a CW-type zinc finger motif, enabling ATP hydrolysis and chromatin interactions. MORC4. specifically, plays roles in DNA repair, cell cycle regulation, and germ cell development, though its precise molecular mechanisms remain under investigation.
Recombinant MORC4 is engineered in vitro using expression systems like *E. coli* or mammalian cells, often tagged with affinity markers (e.g., His, GST) for purification and detection. This engineered protein enables functional studies, including binding assays, enzymatic activity analyses, and exploration of its role in diseases. Dysregulation of MORC4 has been linked to cancers (e.g., breast, pancreatic) and immunological disorders, where it may promote tumorigenesis by modulating pathways like Wnt/β-catenin or impairing DNA damage responses.
Research on recombinant MORC4 also addresses its epigenetic functions, such as chromatin compaction and gene silencing through interactions with histone modifiers. Challenges include clarifying its tissue-specific roles and structural dynamics. Overall, MORC4 recombinant protein serves as a critical tool for unraveling its biological significance and potential therapeutic targeting in disease contexts.
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