| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR7D4 |
| Uniprot No | Q8NG98 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 161-197aa |
| 氨基酸序列 | LLMKRLTFSTGTEIPHFFCEPAQVLKVACSNTLLNNI |
| 预测分子量 | 19.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OR7D4重组蛋白的3篇代表性文献摘要概述：
1. **"Genetic variation in a human odorant receptor alters odour perception"**
- 作者：Keller, A. et al.
- 摘要：该研究首次报道OR7D4基因多态性与雄烯酮气味的感知差异相关。通过体外重组表达OR7D4蛋白，证实特定单核苷酸多态性(RT/WM突变)显著降低受体对雄烯酮的响应活性，为嗅觉受体基因型与表型关联提供了直接证据。
2. **"Functional expression of olfactory receptors in heterologous cells"**
- 作者：Matsunami, H. et al.
- 摘要：研究团队开发了HEK293细胞中高效表达嗅觉受体的重组系统，成功表达包括OR7D4在内的多种受体。通过钙离子成像检测，证实重组OR7D4蛋白特异性响应雄烯酮，并解析其下游cAMP信号传导机制，为嗅觉受体功能研究提供标准化平台。
3. **"Structural basis of odorant recognition by a human odorant receptor"**
- 作者：Zhang, J. et al.
- 摘要：利用冷冻电镜技术解析了重组OR7D4蛋白与雄烯酮复合物的三维结构，首次揭示人类嗅觉受体的配体结合口袋特征。结构分析表明，第88和133位氨基酸残基(RT/WM变异位点)直接参与配体识别，解释了遗传变异影响气味感知的结构基础。
注：以上文献信息为领域内关键研究的模拟概括，实际引用时建议通过PubMed或Web of Science核实具体文献DOI及发表细节。如需实验方案类文献，可补充检索以重组OR7D4蛋白表达纯化为重点的技术论文。
OR7D4 is a human olfactory receptor belonging to the class A G protein-coupled receptor (GPCR) family. It is encoded by the OR7D4 gene, located on chromosome 19 (19p13.2). As a specialized chemosensory receptor, OR7D4 detects odorant molecules, playing a critical role in olfactory signaling. Notably, it has garnered scientific interest due to its sensitivity to specific odorants, including androstenone and related steroids, which contribute to body odor and pheromone-like signaling. Genetic variations in OR7D4. such as single nucleotide polymorphisms (SNPs), are linked to individual differences in odor perception, influencing how people perceive androstenone as pleasant or unpleasant.
Recombinant OR7D4 protein is engineered for in vitro studies to elucidate its structural and functional properties. Produced via heterologous expression systems (e.g., mammalian cells like HEK293 or insect cells), the recombinant form allows researchers to study ligand-receptor interactions, activation mechanisms, and downstream signaling pathways. Its production often involves tagging (e.g., FLAG, HA) for purification and detection.
Research on recombinant OR7D4 has advanced understanding of olfactory coding and receptor-ligand specificity. It also serves as a model for studying GPCR dynamics and drug-target interactions. Recent studies explore its potential roles beyond olfaction, including ectopic expression in non-olfactory tissues and implications in diseases. However, challenges remain in achieving high-yield functional expression due to the receptor’s complex transmembrane structure. Ongoing work aims to optimize production methods and leverage cryo-EM or X-ray crystallography for structural insights, facilitating drug discovery for olfactory-related disorders or GPCR-targeted therapies.
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