| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METTL25 |
| Uniprot No | Q8N6Q8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-603aa |
| 氨基酸序列 | MAASCPLPVTPDLPTLRAKLQGLLQFLRDALSISNAHTVDFYTESVWEELVDLPPETVLAALRKSASETEALPSETRPLVEAEWEAGMTDFPKIFCETSQKLVSVEAFALAAKYYSVQNLGICTPFEQLLVALRGNQNQRIGENQKAVEFMNMKKSHEVQAMSELISSIADYYGIKQVIDLGSGKGYLSSFLSLKYGLKVYGIDSSNTNTHGAEERNRKLKKHWKLCHAQSRLDVNGLALKMAKERKVQNKVKNKADTEEVFNNSPTNQEKMPTSAILPDFSGSVISNIRNQMETLHSQPHQEENLCFENSFSLINLLPINAVEPTSSQQIPNRETSEANKERRKMTSKSSESNIYSPLTSFITADSELHDIIKDLEDCLMVGLHTCGDLAPNTLRIFTSNSEIKGVCSVGCCYHLLSEEFENQHKERTQEKWGFPMCHYLKEERWCCGRNARMSACLALERVAAGQGLPTESLFYRAVLQDIIKDCYGITKCDRHVGKIYSKCSSFLDYVRRSLKKLGLDESKLPEKIIMNYYEKYKPRMNELEAFNMLKVVLAPCIETLILLDRLCYLKEQEDIAWSALVKLFDPVKSPRCYAVIALKKQQ |
| 预测分子量 | 75.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于METTL25重组蛋白的3篇参考文献示例(注：文献为虚构示例，实际研究中请通过学术数据库检索最新成果)：
1. **"METTL25-mediated histone methylation regulates cellular oxidative stress response"**
- Author: Zhang Y. et al.
- 摘要：研究报道了METTL25重组蛋白的体外表达及功能，证实其作为组蛋白甲基转移酶参与氧化应激相关基因的表观遗传调控，并解析了其酶活结构域。
2. **"Structural characterization of recombinant METTL25 and its role in lysine trimethylation"**
- Author: Tanaka K. et al.
- 摘要：通过大肠杆菌系统表达并纯化METTL25重组蛋白，结合晶体结构分析和体外甲基化实验，揭示其对非组蛋白底物中特定赖氨酸残基的修饰机制。
3. **"METTL25 knockout and recombinant protein rescue experiments link mitochondrial function to METTL25 activity"**
- Author: Smith R. et al.
- 摘要：利用重组METTL25蛋白进行功能回补实验，证明其通过调控线粒体相关蛋白的甲基化影响能量代谢通路。
建议通过PubMed或Google Scholar检索关键词“METTL25 recombinant protein”或“METTL25 methyltransferase”获取真实文献。
**Background of METTL25 Recombinant Protein**
METTL25 (Methyltransferase-like 25) is a member of the methyltransferase family, which plays a role in post-translational protein modification by transferring methyl groups to specific substrates. While the METTL family is best known for its involvement in RNA methylation (e.g., METTL3 in mRNA modification), METTL25 is distinguished by its focus on protein targets. Recent studies suggest METTL25 catalyzes lysine methylation, particularly on mitochondrial proteins, influencing their stability, localization, or activity. For example, METTL25 has been linked to the methylation of aldehyde dehydrogenase 1A2 (ALDH1A2), a mitochondrial enzyme involved in retinoic acid synthesis, potentially modulating metabolic pathways or cellular differentiation.
Structurally, METTL25 contains a conserved S-adenosylmethionine (SAM)-binding domain, characteristic of methyltransferases. Recombinant METTL25 protein is typically produced in *E. coli* or mammalian expression systems, often fused with tags (e.g., His-tag) for purification and detection. Its recombinant form enables in vitro studies to dissect enzymatic mechanisms, substrate specificity, and interactions. Researchers also utilize it to explore METTL25’s role in diseases; aberrant methylation by METTL25 has been implicated in cancer progression, neurodegenerative disorders, and metabolic syndromes.
Current research focuses on resolving METTL25’s 3D structure to inform drug design and clarify its regulatory networks. As a relatively understudied enzyme, METTL25 represents a promising target for understanding epigenetic and post-translational modulation of mitochondrial function, with potential therapeutic applications in aging-related and metabolic diseases.
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