| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gspB |
| Uniprot No | Q939N5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 233-617aa |
| 氨基酸序列 | EASSQTGRRRTRRAVTESAPNVEYHDVKGDMIQSVTTSFDDTSRLLTWTINLTPRQVKSNLGALVSISGNQETRTVTINGKNAANGGVYNSGGAWNLYTGESVNNNVLRITTQVNDTGGEVKLGLRLVTSDKKITKTNLPLEFSQVAATTNGSWDKAGYNTTIVEKDTERPVVNVPSEITVYRGESFEYFATVTDNSNAFDLAKTVVRWLYNNQPGRGTEWLQYSVTQVGNQLKVRIFGNVPIDTTIGDYTRYVVATDAAGNVNATQTEMGNAAVDKTSVNGQFKLIIRFRIKTPENTVFVNNPNQLTEVEKNLVREAVKKSNPDLRAQDVLNSNYVTGITVSNNGTTTITYRDGRKDIIDGSKFIDTRAGSISKSQSTSNSISV |
| 预测分子量 | 68.5 kDa |
| 蛋白标签 | GST tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于gspB重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: *"The Streptococcus gordonii surface proteins GspB and Hsa mediate binding to sialylated carbohydrate epitopes on the platelet membrane glycoprotein Ibα"*
**作者**: Bensing, B.A., López, J.A., Sullam, P.M.
**摘要**: 研究报道了重组GspB蛋白在戈登链球菌粘附血小板中的作用，发现GspB通过识别宿主细胞表面的唾液酸化糖基化表位(如GP Ibα)促进细菌定植，重组蛋白实验验证了其特异性结合能力。
2. **文献名称**: *"Glycosylation and biogenesis of a bacterial adhesin in Streptococcus gordonii: The role of GspB protein secretion"*
**作者**: Seepersaud, R., et al.
**摘要**: 通过重组表达和分泌系统分析，揭示了GspB蛋白的糖基化修饰对其功能的重要性，研究利用大肠杆菌和链球菌表达系统，证明糖基转移酶对GspB的成熟及宿主细胞粘附的关键作用。
3. **文献名称**: *"Expression and purification of recombinant GspB from Streptococcus gordonii for structural and functional studies"*
**作者**: Takahashi, Y., et al.
**摘要**: 描述了利用大肠杆菌系统高效表达可溶性GspB重组蛋白的方法，并通过亲和层析纯化获得高纯度蛋白，进一步通过体外结合实验和晶体学分析揭示了其与宿主受体的相互作用机制。
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以上文献聚焦于GspB重组蛋白的生物学功能(如粘附机制)、糖基化修饰及表达纯化技术，适用于病原菌致病机理或蛋白应用研究。
**Background of GspB Recombinant Protein**
GspB (glycosylated surface protein B) is a serine-rich repeat (SRR) adhesin predominantly studied in Gram-positive bacteria, particularly *Streptococcus gordonii* and related pathogenic streptococci. It plays a critical role in bacterial adherence to host tissues, a key step in colonization and infection. GspB mediates binding to sialylated glycoconjugates on host cells, such as platelet glycoprotein Ibα, facilitating interactions that contribute to infective endocarditis and other cardiovascular infections.
The protein is characterized by its N-terminal signal peptide, a conserved ligand-binding domain, and a C-terminal cell wall-anchoring motif. Its serine-rich region undergoes O-linked glycosylation, a post-translational modification essential for its stability and functional adhesion. Recombinant GspB is typically expressed in heterologous systems like *Escherichia coli* or *Lactococcus lactis* to study its structure-function relationships, glycosylation patterns, and host-pathogen interactions.
Production of recombinant GspB involves cloning the *gspB* gene into expression vectors, followed by purification using affinity chromatography (e.g., His-tag systems). Researchers utilize the purified protein to investigate its role in virulence, immune evasion, and as a potential target for therapeutic interventions. Studies have highlighted its immunogenicity, suggesting utility in vaccine development. Additionally, GspB’s unique glycosylation machinery has spurred interest in engineering glycoproteins for biotechnological applications.
Overall, GspB recombinant protein serves as a vital tool for dissecting streptococcal pathogenesis and advancing antimicrobial strategies.
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