| 纯度 | > 90 % SDS-PAGE |
| 种属 | Human |
| 靶点 | ALPP |
| Uniprot No | P05187 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-506aa |
| 氨基酸序列 | IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD |
| 预测分子量 | 54.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与ALPP(胎盘碱性磷酸酶)重组蛋白相关的文献摘要示例(注：文献标题与作者为模拟示例，具体文献需根据实际检索结果调整)：
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1. **文献名称**：Expression and purification of recombinant human placental alkaline phosphatase in HEK293 cells
**作者**：Smith J, et al.
**摘要**：本研究描述了在HEK293哺乳动物细胞中高效表达重组人ALPP蛋白的方法，通过优化转染条件和纯化步骤(如亲和层析)获得高活性蛋白，用于癌症标志物检测研究。
2. **文献名称**：Development of a novel ALPP-based biosensor for early cancer diagnosis
**作者**：Li X, Wang Y, et al.
**摘要**：利用重组ALPP蛋白与纳米材料结合构建生物传感器，实现了对卵巢癌和睾丸癌患者血清中低浓度ALPP的高灵敏度检测，临床样本验证显示显著诊断价值。
3. **文献名称**：Structural and functional analysis of ALPP mutants in pre-eclampsia studies
**作者**：Chen R, et al.
**摘要**：通过原核系统(大肠杆菌)表达重组ALPP突变体，分析其酶活性变化，揭示特定氨基酸位点异常与妊娠期高血压疾病的潜在关联。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“recombinant ALPP”、“placental alkaline phosphatase expression”等获取最新研究。
**Background of ALPP Recombinant Protein**
ALPP (Alkaline Phosphatase, Placental-like), also known as placental alkaline phosphatase (PLAP), is a glycosylphosphatidylinositol (GPI)-anchored membrane protein belonging to the alkaline phosphatase (ALP) family. This enzyme catalyzes the hydrolysis of phosphate esters in an alkaline environment, playing roles in mineralization, metabolism, and detoxification. ALPP is primarily expressed in the placenta during pregnancy but is also ectopically produced in certain cancers, including ovarian, testicular, and lung malignancies, making it a potential biomarker for tumor detection and monitoring.
Recombinant ALPP is produced using genetic engineering techniques, where the *ALPP* gene is cloned into expression vectors and transfected into host systems like mammalian cells (e.g., HEK293) or insect cells. This approach ensures high purity, specificity, and scalability compared to native sources. The recombinant protein retains enzymatic activity and structural integrity, enabling applications in research, diagnostics, and therapeutics.
In biomedical research, recombinant ALPP serves as a tool to study enzyme kinetics, cellular signaling, and cancer biology. It is widely used in immunoassays (e.g., ELISA, Western blot) as a detection label due to its stable activity and compatibility with colorimetric substrates. Additionally, ALPP's overexpression in tumors has spurred interest in developing antibody-drug conjugates (ADCs) or CAR-T therapies targeting ALPP-positive cancers.
Despite its utility, challenges remain in optimizing post-translational modifications (e.g., glycosylation) for functional consistency across host systems. Ongoing studies aim to refine production methods and explore ALPP's role in disease mechanisms, underscoring its dual significance as a diagnostic marker and therapeutic target.
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