| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC174 |
| Uniprot No | Q6PII3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-467aa |
| 氨基酸序列 | MDRRKKPLDVTASSLVDLKAELFRKQEEFKQEKLLKDSGVFGKPKTTNKKPSIWSKQNVGVSNRAEKDAEQKIEEQKTLDKAREKLEEKAKLYEKMTKGDFIDEEVEDMYLVDFTQKIIDKRKEMEASGAHRDSQKAGERDDDEENLPEGEIPPPQDPSEEWVDYVDSLGRSRRCMRKDLPDLLEMDKNLQGRLFISPANEKTLLSEDMRKELQRQQWEEEEREALKRPMGPVHYEDIRENEARQLGVGYFAFARDKELRNKQMKTLEMLREQTTDQRTKRENIKEKRKAILEARLAKLRQKKMKKSKEGGTEEENRDGDVIGPLPPEPEAVPTPRPAAQSSKVEVIVQERKDTKPGVPHIREWDRGKEFSFGYWSKRQSDLRAERDPEFAPPSDYFVGQKRTGFSSSQAWSRPGPAQSDPGQCPDQSHGPSPEHTSPTPAPDNPPQAPTVTFKTLDDMISYYKQVT |
| 预测分子量 | 59.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的参考文献为基于CCDC174相关研究的假设性示例，实际文献可能需要通过学术数据库进一步检索确认：
1. **文献名称**：*CCDC174 Recombinant Protein Expression and Its Role in Cell Cycle Regulation*
**作者**：Zhang L., et al.
**摘要**：本研究成功构建了CCDC174的重组表达载体，并在HEK293细胞中高效表达。通过体外实验证实，重组CCDC174蛋白通过与细胞周期相关激酶相互作用，调控G2/M期转换，为探究其在肿瘤发生中的作用提供了依据。
2. **文献名称**：*Structural Characterization of Human CCDC174 Using Recombinant Protein Technology*
**作者**：Kim S., Park J.H.
**摘要**：利用大肠杆菌表达系统纯化获得高纯度CCDC174重组蛋白，结合X射线晶体学解析其三维结构，发现其具有独特的卷曲螺旋结构域，可能参与蛋白质互作网络，为功能研究奠定结构基础。
3. **文献名称**：*CCDC174 Recombinant Protein as a Potential Biomarker in Male Infertility*
**作者**：Wang Y., et al.
**摘要**：通过原核表达获得CCDC174重组蛋白，并制备多克隆抗体。临床样本分析显示，CCDC174在少精症患者精子中表达显著下调，提示其可能作为生殖功能障碍的诊断标志物。
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**备注**：若实际文献稀缺，建议扩大检索范围至CCDC家族蛋白的功能研究，或关注基因敲除/敲低实验的文献。使用PubMed/Google Scholar时，可尝试关键词："CCDC174 recombinant expression"、"CCDC174 protein function"。
CCDC174 (Coiled-coil domain-containing protein 174) is a less characterized protein encoded by the CCDC174 gene, which is conserved across vertebrates and located on human chromosome 3. The protein is named for its prominent structural feature: multiple coiled-coil domains, which are helical motifs mediating protein-protein interactions. These domains suggest a potential role in scaffolding or organizing multi-protein complexes, though its precise molecular functions remain under investigation. Preliminary studies indicate that CCDC174 may participate in cellular processes such as cell cycle regulation, cytoskeletal organization, or intracellular transport, given its structural similarity to other coiled-coil proteins involved in these pathways.
Recombinant CCDC174 protein is artificially produced in heterologous expression systems (e.g., E. coli, HEK293 cells) for functional studies. Its recombinant form enables researchers to explore biochemical properties, interaction partners, and post-translational modifications in controlled settings. Emerging evidence links CCDC174 to human diseases. For example, transcriptomic analyses show altered CCDC174 expression in certain cancers, including hepatocellular carcinoma and gliomas, implying a potential role in tumorigenesis. Additionally, gene variants near CCDC174 loci have been associated with neurodevelopmental disorders, though causality remains unconfirmed.
Current research focuses on elucidating its subcellular localization (possibly nuclear or cytoskeletal), interactome networks, and signaling pathways. Challenges include the lack of specific antibodies and animal models, which recombinant protein tools aim to address. By enabling structural studies (e.g., X-ray crystallography) and antibody development, recombinant CCDC174 accelerates mechanistic insights into its biological roles and disease relevance. Further characterization may reveal therapeutic targets or diagnostic biomarkers, particularly in oncology and neurology.
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