| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB12 |
| Uniprot No | Q6IQ22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-244aa |
| 氨基酸序列 | MDPGAALQRRAGGGGGLGAGSPALSGGQGRRRKQPPRPADFKLQVIIIGSRGVGKTSLMERFTDDTFCEACKSTVGVDFKIKTVELRGKKIRLQIWDTAGQERFNSITSAYYRSAKGIILVYDITKKETFDDLPKWMKMIDKYASEDAELLLVGNKLDCETDREITRQQGEKFAQQITGMRFCEASAKDNFNVDEIFLKLVDDILKKMPLDILRNELSNSILSLQPEPEIPPELPPPRPHVRCC |
| 预测分子量 | 56.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB12重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**: *"RAB12 regulates autophagy in skeletal muscle through its interaction with the ULK1 complex"*
**作者**: Smith J, et al.
**摘要**: 本研究揭示了RAB12通过直接与自噬起始复合物ULK1相互作用，调控骨骼肌细胞中自噬小体的形成。重组RAB12蛋白的体外实验表明，其GTP酶活性对自噬通量具有动态调节作用。
2. **文献名称**: *"Recombinant RAB12 characterization and its role in vesicular trafficking"*
**作者**: Lee S, Kim D.
**摘要**: 作者通过大肠杆菌系统表达并纯化了重组RAB12蛋白，发现其参与调控早期内体到高尔基体的逆向运输，并证明其磷酸化修饰影响与效应蛋白的相互作用。
3. **文献名称**: *"RAB12-mediated lysosomal degradation of EGFR in non-small cell lung cancer"*
**作者**: Chen X, et al.
**摘要**: 该研究利用重组RAB12蛋白进行功能验证，发现RAB12通过促进表皮生长因子受体(EGFR)的溶酶体降解，抑制非小细胞肺癌的增殖，为靶向治疗提供了新思路。
注：以上文献信息为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索获取。
**Background of RAB12 Recombinant Protein**
RAB12 is a member of the RAB family of small GTPases, which play critical roles in regulating intracellular membrane trafficking, vesicle transport, and organelle dynamics. As a GTP-binding protein, RAB12 cycles between an active GTP-bound state and an inactive GDP-bound state, a process tightly controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). RAB12 is predominantly localized to the Golgi apparatus, endosomes, and autophagosomes, where it participates in cargo sorting, lysosomal degradation, and autophagy-related pathways.
Studies suggest that RAB12 is involved in unconventional secretion pathways, mitochondrial quality control, and the regulation of cell surface receptor internalization. Dysregulation of RAB12 has been linked to neurodegenerative diseases, cancer progression, and lysosomal storage disorders, highlighting its physiological and pathological significance. For instance, RAB12 overexpression in certain tumors correlates with enhanced cell proliferation and metastatic potential.
Recombinant RAB12 protein is engineered for in vitro studies to dissect its molecular interactions, enzymatic activity, and role in cellular processes. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cell lines), the recombinant protein retains functional domains required for GTP hydrolysis and effector binding. It is often fused with tags (e.g., GST, His-tag) to facilitate purification and detection. Applications include biochemical assays (e.g., GTPase activity measurement), structural studies, and screening for RAB12-targeting therapeutics. Recent research also explores its involvement in autophagy-lysosomal dysfunction in conditions like Parkinson’s disease, making recombinant RAB12 a valuable tool for both basic and translational investigations.
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