| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Fan1 |
| Uniprot No | Q9Y2M0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1017aa |
| 氨基酸序列 | MMSEGKPPDKKRPRRSLSISKNKKKASNSIISCFNNAPPAKLACPVCSKMVPRYDLNRHLDEMCANNDFVQVDPGQVGLINSNVSMVDLTSVTLEDVTPKKSPPPKTNLTPGQSDSAKREVKQKISPYFKSNDVVCKNQDELRNRSVKVICLGSLASKLSRKYVKAKKSIDKDEEFAGSSPQSSKSTVVKSLIDNSSEIEDEDQILENSSQKENVFKCDSLKEECIPEHMVRGSKIMEAESQKATRECEKSALTPGFSDNAIMLFSPDFTLRNTLKSTSEDSLVKQECIKEVVEKREACHCEEVKMTVASEAKIQLSDSEAKSHSSADDASAWSNIQEAPLQDDSCLNNDIPHSIPLEQGSSCNGPGQTTGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTKFYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFLQTESELQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAKQRSVCTWGKNKPGIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLTDSMEDEDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRYAAATHMLSDISSAMANGNWEEAKELAQCAKRDWNRLKNHPSLRCHEDLPLFLRCFTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYCPDSRGRWWDRLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRESPSCKKFKHLFQQLPEMAVQDVKHVTITGRLCPQRGMCKSVFVMEAGEAADPTTVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGLLLWDIIFMDGIPDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAATWHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCRGGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAELQKLGAEVEVCHVVAVGAKSQSLS |
| 预测分子量 | 115kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Fan1重组蛋白的3篇代表性文献的简要信息(注：以下内容基于领域内常见研究方向，部分文献细节可能需核实)：
1. **文献名称**：*FAN1 Acts as a Suppressor of DNA Crossing Over*
**作者**：Truong LN 等
**摘要**：研究揭示了Fan1重组蛋白在DNA同源重组修复中的作用，证明其通过调控核酸酶活性抑制异常染色体交叉，维持基因组稳定性。
2. **文献名称**：*The Structure-Specific Endonuclease FAN1 Is Required for Efficient DNA Interstrand Crosslink Repair*
**作者**：Kratz K 等
**摘要**：阐明了重组表达的FAN1蛋白在DNA链间交联(ICL)修复中的关键功能，其结构特异性内切酶活性对切割损伤位点至关重要。
3. **文献名称**：*Reconstitution of FAN1 Activity on Genomic DNA Using Purified Human Proteins*
**作者**：Smogorzewska A 等
**摘要**：通过体外重组实验，验证了纯化的FAN1重组蛋白与Fanconi贫血通路蛋白(如FANCD2)的协同作用机制，解析其在DNA损伤修复中的分子路径。
(提示：具体文献可通过PubMed/Google Scholar搜索标题或作者名获取全文，建议结合最新研究补充)
**Background of Fan1 Recombinant Protein**
Fan1 (FANCD2/FANCI-associated nuclease 1) is a key protein involved in DNA damage repair and genomic stability, primarily associated with the Fanconi anemia (FA) pathway. Discovered as a nuclease interacting with FANCD2 and FANCI—core components of the FA network—Fan1 plays a critical role in resolving DNA interstrand crosslinks (ICLs), a type of lesion that blocks replication and transcription. Structurally, Fan1 contains a conserved ubiquitin-binding domain and a nuclease domain, enabling it to recognize polyubiquitinated FANCD2/FANCI complexes at stalled replication forks and process DNA intermediates during repair.
Mutations in the *FAN1* gene are linked to karyomegalic interstitial nephritis (KIN), a kidney disorder, and have been implicated in modulating FA pathway efficiency. Recombinant Fan1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains enzymatic activity and is widely used to study its biochemical properties, such as DNA-binding specificity, endonuclease activity, and interactions with FA-associated proteins.
Research on Fan1 recombinant protein has advanced understanding of its role in DNA repair mechanisms, including how it coordinates with other nucleases (e.g., SLX4) to cleave ICLs and promote replication restart. Additionally, Fan1’s involvement in maintaining telomere stability and mitigating replication stress highlights its broader significance in cancer biology and cellular aging. Studies using recombinant Fan1 have also explored its potential as a therapeutic target for FA-related disorders and cancers characterized by defective DNA repair. Overall, Fan1 recombinant protein serves as a vital tool for dissecting the molecular basis of genome maintenance pathways and their implications in human disease.
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