| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Gsdma3 |
| Uniprot No | Q5Y4Y6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 263-464aa |
| 氨基酸序列 | MHEDFKTLKEEVQRETQEVEKLSPVGRSSLLTSLSHLLGKKKELQDLEQKLEGALDKGQKVTLEALPKDVLLSKDAMDAILYFLGALTELTEEQLKILVKSLEKKILPVQLKLVESTLEQNFLQDKEGVFPLQPDLLSSLGEEELTLTEALVGLSGLEVQRSGPQYAWDPDTRHNLCALYAGLSLLHLLSRKSNALTYCALS |
| 预测分子量 | 26.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Gsdma3重组蛋白的3篇参考文献概览：
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1. **文献名称**：*Gasdermin A3 regulates hair follicle differentiation via modulating nuclear factor-κB signaling in keratinocytes*
**作者**：Zhou Y, et al.
**摘要**：该研究通过构建Gsdma3重组蛋白，揭示其在毛囊分化中的作用机制。实验表明，Gsdma3通过调控NF-κB信号通路影响角质形成细胞的增殖与凋亡，其功能缺失导致小鼠皮肤屏障异常及脱毛表型。
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2. **文献名称**：*Structural insights into the oligomerization of Gsdma3 and its role in pyroptosis*
**作者**：Li P, et al.
**摘要**：通过解析Gsdma3重组蛋白的晶体结构，发现其N端结构域可形成寡聚体并诱导细胞膜穿孔，触发焦亡。研究阐明了Gsdma3激活焦亡的结构基础，为炎症性皮肤疾病的治疗提供新靶点。
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3. **文献名称**：*Gsdma3-driven inflammasome activation contributes to psoriasis-like skin inflammation in mice*
**作者**：Wang K, et al.
**摘要**：利用Gsdma3重组蛋白过表达模型，证明其通过激活NLRP3炎症小体促进IL-1β释放，导致小鼠银屑病样皮肤炎症。研究揭示了Gsdma3在自身免疫性疾病中的病理机制。
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**注**：以上文献为虚拟示例，实际研究中建议通过PubMed或Google Scholar检索具体论文。关键词建议：Gsdma3. gasdermin, pyroptosis, recombinant protein, skin inflammation.
Gsdma3. a member of the gasdermin protein family, plays a critical role in regulating cell death and inflammatory responses. The gasdermin family is characterized by a conserved N-terminal pore-forming domain that executes pyroptosis, a form of programmed lytic cell death linked to immune defense and disease pathogenesis. Gsdma3. specifically, is predominantly expressed in epithelial tissues, particularly the skin and hair follicles, and has been implicated in maintaining epidermal homeostasis. Mutations in the Gsdma3 gene are associated with skin inflammation, alopecia, and other epithelial disorders in mouse models, highlighting its importance in tissue integrity and immune regulation.
Structurally, Gsdma3 contains two key domains: an N-terminal pore-forming domain and a C-terminal autoinhibitory domain. Under physiological conditions, these domains remain bound, keeping the protein inactive. Upon activation—often triggered by proteolytic cleavage or cellular stress—the N-terminal domain is released, oligomerizes, and forms pores in the cell membrane, leading to pyroptosis. This process facilitates the release of pro-inflammatory cytokines, amplifying immune responses but also contributing to tissue damage if dysregulated.
Recombinant Gsdma3 protein is engineered to study its biochemical properties, activation mechanisms, and interactions with other cellular components. Produced typically in Escherichia coli or mammalian expression systems, the recombinant protein retains functional domains and is used in vitro to dissect pore-forming activity, lipid-binding specificity, and regulatory pathways. Researchers employ techniques like liposome assays, cellular transfection, and CRISPR-mediated gene editing to explore its role in disease models, particularly skin inflammation and cancer. Additionally, recombinant Gsdma3 aids in developing inhibitors targeting gasdermin-mediated pyroptosis, offering potential therapeutic strategies for inflammatory diseases. Its study bridges gaps in understanding how epithelial tissues balance cell survival, death, and immune signaling, making it a focal point in both basic and translational research.
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