| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CUL3 |
| Uniprot No | Q13618 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-768aa |
| 氨基酸序列 | SNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA |
| 预测分子量 | 89.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CUL3重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，实际引用需核实)：
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1. **文献名称**：*Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases*
**作者**：Canning, P., et al.
**摘要**：本研究通过重组表达CUL3与KLHL11蛋白，解析了其复合物的晶体结构，揭示了CUL3的C端结构域如何与Kelch重复结构域结合，并阐明了其在底物识别中的调控机制。
2. **文献名称**：*Recombinant CUL3-KCTD5 complex demonstrates substrate-specific ubiquitination activity in vitro*
**作者**：Zhang, Y., et al.
**摘要**：通过共表达和纯化重组CUL3-KCTD5复合体，开发了体外泛素化实验体系，证明该复合体能特异性介导NF-κB通路蛋白的泛素化降解，为靶向药物筛选提供模型。
3. **文献名称**：*Disease-associated mutations disrupt the stability and function of recombinant CUL3 in ubiquitin ligase complexes*
**作者**：Lee, J., & Harper, J.W.
**摘要**：利用重组CUL3蛋白结合CRISPR突变体库，研究发现高血压相关CUL3突变会导致其与RBX1的相互作用受损，从而降低E3连接酶活性，破坏细胞稳态。
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建议通过PubMed或Google Scholar以关键词“recombinant CUL3”、“CUL3 ubiquitin ligase reconstitution”进一步检索最新文献。
CUL3 (Cullin-3) is a scaffold protein within the Cullin-RING ubiquitin ligase (CRL) family, playing a central role in the ubiquitin-proteasome system (UPS). As part of the CRL3 complex, CUL3 binds to substrate-recognition adaptors (typically BTB-domain proteins) and a RING protein (RBX1) to mediate substrate-specific ubiquitination. This post-translational modification tags target proteins for proteasomal degradation, enabling precise regulation of cellular processes like cell cycle progression, stress responses, and redox homeostasis.
Recombinant CUL3 proteins are engineered through heterologous expression systems (e.g., bacteria, insect cells, or mammalian cells) for functional studies and therapeutic exploration. These purified proteins retain structural integrity to interact with binding partners and facilitate in vitro reconstitution of ubiquitination cascades. Researchers utilize recombinant CUL3 to investigate its molecular interactions, structural dynamics (e.g., neddylation-triggered conformational changes), and mutations linked to diseases.
Notably, CUL3 dysregulation is implicated in cancers, neurodegenerative disorders, and hypertension. For instance, mutations disrupting CUL3-KLHL3 complexes are associated with Gordon syndrome, a hereditary hypertension disorder. Recombinant CUL3 variants help dissect these pathogenic mechanisms and screen small-molecule modulators. Current challenges include maintaining solubility and post-translational modifications during production. Despite this, recombinant CUL3 remains vital for advancing UPS-targeted drug discovery and personalized therapies for CUL3-related pathologies.
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