| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OVOL3 |
| Uniprot No | O00110 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-214aa |
| 氨基酸序列 | MPRAFLVRSRRPQPPNWGHLPDQLRGDAYIPGGPLTVPGGKGQERRSVTIWLFSSDCSSLGGPPAQQSSSVRDPWTAQPTQGNLTSAPRGPGTLGCPLCPKAFPLQRMLTRHLKCHSPVRRHLCRCCGKGFHDAFDLKRHMRTHTGIRPFRCSACGKAFTQRCSLEAHLAKVHGQPASYAYRERREKLHVCEDCGFTSSRPDTYAQHRALHRAA |
| 预测分子量 | 31.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OVOL3重组蛋白的3篇参考文献的示例(注：以下内容为模拟生成，仅供参考)：
1. **"Functional characterization of recombinant OVOL3 protein in epithelial-mesenchymal transition"**
- 作者：Zhang Y, et al.
- 摘要：该研究通过构建并纯化重组OVOL3蛋白，发现其通过抑制ZEB1等转录因子调控上皮-间质转化(EMT)，揭示了OVOL3在维持上皮细胞表型中的关键作用。
2. **"OVOL3 recombinant protein modulates stem cell differentiation via Wnt/β-catenin signaling"**
- 作者：Lee S, et al.
- 摘要：研究团队利用重组OVOL3蛋白进行体外实验，证明其通过抑制Wnt/β-catenin信号通路影响胚胎干细胞的定向分化，提示OVOL3在发育过程中的调控功能。
3. **"Structural analysis of OVOL3 DNA-binding domain reveals conserved mechanisms in Ovo-like family"**
- 作者：Chen X, et al.
- 摘要：通过重组表达并结晶OVOL3蛋白的DNA结合结构域，研究发现其锌指结构与OVOL家族成员(如OVOL1/2)高度保守，为理解其转录抑制机制提供了结构基础。
4. **"Recombinant OVOL3 protein suppresses tumor metastasis by targeting TGF-β signaling"**
- 作者：Wang H, et al.
- 摘要：该文献报道重组OVOL3蛋白在癌细胞模型中通过拮抗TGF-β/Smad通路抑制侵袭和转移，为癌症治疗提供了潜在靶点。
(注：实际文献需通过学术数据库验证。建议使用PubMed、Web of Science等平台以关键词“OVOL3 recombinant”检索最新研究。)
OVOL3 (Ovo-Like Transcriptional Repressor 3) is a member of the OVOL family of transcription factors, which are evolutionarily conserved regulators of cellular differentiation and development. The OVOL family, including OVOL1. OVOL2. and OVOL3. plays critical roles in epithelial cell fate determination, epithelial-mesenchymal transition (EMT), and tissue morphogenesis. OVOL3. specifically, is less characterized compared to OVOL1 and OVOL2 but shares structural features such as a conserved zinc-finger DNA-binding domain, enabling sequence-specific interactions with target gene promoters. It functions primarily as a transcriptional repressor, modulating genes involved in cell cycle progression, differentiation, and EMT.
Research suggests OVOL3 is implicated in maintaining epithelial cell identity by suppressing mesenchymal traits, thereby influencing tissue homeostasis and repair. Its expression is detected in various epithelial tissues, including the skin, gut, and reproductive organs. Dysregulation of OVOL3 has been linked to pathological conditions, particularly cancers, where it may act as a tumor suppressor by inhibiting pro-metastatic pathways. For instance, reduced OVOL3 expression correlates with increased invasiveness in certain carcinomas, highlighting its role in restraining EMT-driven metastasis.
Recombinant OVOL3 protein is engineered for in vitro and in vivo studies to elucidate its molecular mechanisms. Produced typically in bacterial or mammalian expression systems, the recombinant protein retains functional domains necessary for DNA binding and transcriptional regulation. It is utilized in assays such as electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and cell-based reporter systems to identify target genes and signaling pathways. Additionally, it aids in structural studies to map interaction interfaces with co-regulators or chromatin modifiers. As a tool, recombinant OVOL3 enables exploration of its therapeutic potential in cancer, fibrosis, and regenerative medicine, offering insights into strategies to manipulate epithelial plasticity for disease intervention.
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