| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rv3304 |
| Uniprot No | O53356 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159aa |
| 氨基酸序列 | MPLYAAYGSNMHPEQMLERAPHSPMAGTGWLPGWRLTFGGEDIGWEGALATVVEDPDSKVFVVLYDMTPADEKNLDRWEGSEFGIHQKIRCRVERISSDTTTDPVLAWLYVLDAWEGGLPSARYLGVMADAAEIAGAPSDYVHDLRTRPARNIGPGTIA |
| 预测分子量 | 24.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rv3304重组蛋白的3篇参考文献示例(注：内容为示例性概括，具体文献需通过学术数据库验证)：
1. **文献名称**：*Immunogenicity and protective efficacy of recombinant Rv3304 protein against Mycobacterium tuberculosis in mice*
**作者**：Zhang, T., Li, S., et al.
**摘要**：研究通过在大肠杆菌中表达Rv3304重组蛋白，评估其在小鼠模型中的免疫原性和保护效果，结果显示该蛋白可诱导Th1型免疫反应并降低结核分枝杆菌负荷。
2. **文献名称**：*Cloning and characterization of Rv3304: A potential diagnostic antigen for tuberculosis*
**作者**：Wang, Y., Chen, X., et al.
**摘要**：报道了Rv3304基因的克隆、重组表达及纯化，并通过血清学实验证明其在结核病患者血清中具有较高特异性抗体反应，提示其作为诊断标志物的潜力。
3. **文献名称**：*Structural and functional analysis of Rv3304 from Mycobacterium tuberculosis*
**作者**：Kumar, A., Singh, A., et al.
**摘要**：通过X射线晶体学解析了Rv3304蛋白的三维结构，结合体外酶活实验，揭示了其可能参与分枝杆菌细胞壁代谢的生物学功能。
建议通过PubMed或Web of Science以“Rv3304 recombinant protein”、“Mycobacterium tuberculosis Rv3304”等关键词检索获取具体文献。
**Background of Rv3304 Recombinant Protein**
The Rv3304 gene, encoded by *Mycobacterium tuberculosis* (Mtb), is associated with the pathogen’s survival and virulence mechanisms. This gene is annotated as a probable conserved transmembrane protein, though its exact biological function remains under investigation. Rv3304 is speculated to play a role in lipid metabolism or cell wall biosynthesis, critical processes for Mtb’s resilience in hostile host environments. Its expression is linked to stress responses, suggesting involvement in adapting to host immune pressures.
Recombinant Rv3304 protein is produced via genetic engineering, typically by cloning the Rv3304 gene into expression vectors (e.g., *E. coli*) and purifying the protein for research applications. This approach enables large-scale production of the antigen for functional and immunological studies. Studies highlight Rv3304’s potential as a diagnostic or vaccine candidate due to its immunogenicity. For instance, it elicits specific antibody and T-cell responses in exposed individuals, distinguishing infected from vaccinated hosts in some assays.
Research also explores Rv3304’s role in host-pathogen interactions. Structural analyses reveal conserved domains that may interact with host immune components, though mechanistic details are unclear. Challenges include clarifying its physiological role, optimizing recombinant protein stability, and validating its utility in TB diagnostics or subunit vaccines. Current efforts focus on integrating Rv3304 into multi-antigen panels to improve sensitivity/specificity in TB detection or prevention. Overall, Rv3304 represents a promising yet underexplored target for addressing tuberculosis, a global health priority.
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