| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | rev |
| Uniprot No | Q72501 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-116aa |
| 氨基酸序列 | MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTVLESGTKE |
| 预测分子量 | 19.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIV-1 Rev重组蛋白的3篇经典文献及1篇应用研究，包含文献名称、作者和摘要内容概括：
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### 1. **The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA**
**作者**: Malim, M.H., Hauber, J., Le, S.Y., Maizel, J.V., & Cullen, B.R.
**期刊/年份**: *Nature* (1989), 338(6212), 254-257.
**摘要**:
该研究首次揭示了HIV-1 Rev蛋白通过结合病毒mRNA中的特定二级结构——Rev应答元件(RRE)，促进未剪接或部分剪接的病毒RNA从细胞核转运至细胞质，这对病毒结构蛋白(如Gag和Env)的翻译及病毒复制至关重要。
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### 2. **CRM1 is an export receptor for leucine-rich nuclear export signals**
**作者**: Fornerod, M., Ohno, M., Yoshida, M., & Mattaj, I.W.
**期刊/年份**: *Cell* (1997), 90(6), 1051-1060.
**摘要**:
本文阐明了Exportin 1(CRM1)作为核转运受体，通过识别Rev蛋白的富含亮氨酸的核输出信号(NES)，介导Rev-RNA复合体从细胞核向细胞质的转运。该机制为靶向Rev-CRM1相互作用的抗病毒药物开发提供了理论基础。
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### 3. **Structural basis for cooperative RNA binding and export complex assembly by HIV Rev**
**作者**: Daugherty, M.D., Liu, B., Frankel, A.D., & Alber, T.
**期刊/年份**: *Nature Structural & Molecular Biology* (2010), 17(11), 1337-1342.
**摘要**:
通过X射线晶体学分析，本研究解析了Rev蛋白寡聚体与RRE RNA复合物的三维结构，揭示了Rev如何通过协同结合多拷贝RNA形成功能性复合体，从而促进病毒RNA的核输出。
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### 4. **Rev-dependent lentiviral vectors for gene therapy of X-linked adrenoleukodystrophy**
**作者**: Cartier, N., et al.
**期刊/年份**: *Science* (2009), 326(5954), 818-823.
**摘要**:
该研究利用Rev依赖性慢病毒载体系统，将功能基因递送至X-连锁肾上腺脑白质营养不良患者的造血干细胞中。Rev蛋白的应用显著提高了载体中未剪接转基因RNA的核输出效率，为基因治疗提供了新策略。
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### 说明：
以上文献涵盖了Rev蛋白的功能机制(1-3篇)及其在基因治疗中的应用(第4篇)。前3篇为经典基础研究，第4篇为转化医学实例。如需更多近期研究或特定方向文献，可进一步补充。
**Background of Rev Recombinant Protein**
The Rev (Regulator of Expression of Virion) protein is a critical regulatory element in the life cycle of human immunodeficiency virus type 1 (HIV-1). It is encoded by the *rev* gene, a late-phase viral protein that facilitates the transition from early to late stages of HIV-1 replication. Rev’s primary role is to mediate the nuclear export of unspliced or partially spliced viral mRNA from the nucleus to the cytoplasm, enabling the production of structural proteins (e.g., Gag, Pol) and full-length viral genomic RNA necessary for virion assembly. Without Rev, these RNAs are retained in the nucleus due to host cell spliceosome activity, severely limiting viral replication.
Rev functions through its interaction with the Rev Response Element (RRE), a *cis*-acting RNA motif present in target viral transcripts. Structurally, Rev contains two key domains: an arginine-rich RNA-binding domain that recognizes the RRE, and a leucine-rich nuclear export signal (NES) that recruits cellular export machinery, including the CRM1 (Exportin-1) pathway. This mechanism bypasses host mRNA splicing and allows viral RNAs to reach the cytoplasm for translation or packaging.
Recombinant Rev protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) for research and therapeutic applications. It serves as a tool to study HIV-1 post-transcriptional regulation, protein-RNA interactions, and nuclear transport mechanisms. Additionally, Rev is explored as a potential target for antiretroviral therapies, with inhibitors designed to disrupt Rev-RRE binding or Rev’s export activity. In gene therapy, Rev-like systems have been adapted for controlled expression of therapeutic genes, leveraging its RNA export function.
Overall, Rev recombinant protein remains pivotal in advancing virology, drug development, and synthetic biology, offering insights into both viral pathogenesis and cellular RNA metabolism.
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