| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDPK1 |
| Uniprot No | Q9BJF5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-507aa |
| 氨基酸序列 | MGQQESTLGGAAGEPRSRGHAAGTSGGPGDHLHATPGMFVQHSTAIFSDRYKGQRVLGKGSFGEVILCKDKITGQECAVKVISKRQVKQKTDKESLLREVQLLKQLDHPNIMKLYEFFEDKGYFYLVGEVYTGGELFDEIISRKRFSEVDAARIIRQVLSGITYMHKNKIVHRDLKPENLLLESKSKDANIRIIDFGLSTHFEASKKMKDKIGTAYYIAPEVLHGTYDEKCDVWSTGVILYILLSGCPPFNGANEYDILKKVEKGKYTFELPQWKKVSESAKDLIRKMLTYVPSMRISARDALDHEWIQTYTKEQISVDVPSLDNAILNIRQFQGTQKLAQAALLYMGSKLTSQDETKELTAIFHKMDKNGDGQLDRAELIEGYKELMRMKGQDASMLDASAVEHEVDQVLDAVDFDKNGYIEYSEFVTVAMDRKTLLSRERLERAFRMFDSDNSGKISSTELATIFGVSDVDSETWKSVLSEVDKNNDGEVDFDEFQQMLLKLCGN |
| 预测分子量 | 63.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDPK1重组蛋白的3篇参考文献示例(内容基于已有研究领域知识整合，非真实文献，仅供参考)：
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1. **标题**: *"Functional characterization of Plasmodium falciparum CDPK1 recombinant protein in calcium-dependent signaling"*
**作者**: Billker, O. et al.
**摘要**: 通过大肠杆菌表达系统成功获得重组PfCDPK1蛋白，体外酶活实验证实其依赖钙离子激活，并磷酸化特定底物，提示其在疟原虫入侵红细胞阶段的信号转导中起关键作用。
2. **标题**: *"Structural insights into CDPK1 from Toxoplasma gondii using recombinant protein crystallography"*
**作者**: Siden-Kiamos, I. et al.
**摘要**: 利用昆虫细胞表达系统纯化TgCDPK1重组蛋白，通过X射线晶体学解析其三维结构，揭示钙结合域与激酶域的构象变化机制，为设计靶向抑制剂提供结构基础。
3. **标题**: *"High-throughput screening of CDPK1 inhibitors using a recombinant kinase assay platform"*
**作者**: Green, J.L. et al.
**摘要**: 建立基于重组CDPK1蛋白的体外激酶活性检测体系，筛选出多个小分子抑制剂，其中化合物A显著抑制疟原虫体外生长，验证CDPK1作为抗寄生虫药物靶点的潜力。
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注：以上为模拟文献，实际文献需通过PubMed/Google Scholar检索关键词“CDPK1 recombinant protein”获取。
CDPK1 (Calcium-Dependent Protein Kinase 1) is a serine/threonine kinase that plays a critical role in calcium-mediated signaling pathways, particularly in protozoan parasites such as *Plasmodium* species (malaria parasites) and *Toxoplasma gondii*. It belongs to the CDPK family, which uniquely combines kinase activity with calcium-sensing domains, enabling direct activation by calcium flux—a mechanism distinct from classical calmodulin-dependent kinases. CDPK1 is essential for key life cycle stages of these pathogens, including host cell invasion, gliding motility, and egress. In *Plasmodium*, for example, CDPK1 regulates microneme secretion, a process vital for parasite motility and host cell penetration.
Recombinant CDPK1 protein is produced through heterologous expression systems (e.g., *E. coli*, insect cells) to study its structure, enzymatic activity, and interactions with inhibitors. Its recombinant form retains calcium-dependent autophosphorylation and substrate phosphorylation capabilities, making it a valuable tool for drug discovery. Researchers use it to screen small-molecule inhibitors that could block parasite-specific signaling without affecting human kinases, offering potential for antimalarial or antiparasitic therapies. Structural studies of recombinant CDPK1 have revealed ATP-binding pockets and regulatory domains as targets for rational drug design.
Despite progress, challenges remain in achieving selective inhibition due to structural similarities between parasitic and mammalian kinases. Ongoing research focuses on optimizing inhibitor specificity and understanding resistance mechanisms. Recombinant CDPK1 continues to bridge gaps in parasitology and kinase biology, highlighting its dual role as a mechanistic model and therapeutic target.
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