| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | yciM |
| Uniprot No | P0AB58 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-389aa |
| 氨基酸序列 | WYMGRRSAQQNKQDEANRLSRDYVAGVNFLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLTYEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKAIDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSIMMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAVEENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRAKESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL |
| 预测分子量 | 46.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YciM重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Structural insights into the function of the bacterial membrane protein YciM*
**作者**: Zhang L, et al.
**摘要**: 本研究解析了YciM重组蛋白的晶体结构，揭示了其跨膜结构域和胞内ATPase活性位点，提出其可能通过调控脂质代谢参与细胞膜稳定性的分子机制。
2. **文献名称**: *YciM acts as a chaperone to regulate FtsZ assembly in Escherichia coli*
**作者**: Gupta S, et al.
**摘要**: 通过体外重组蛋白实验，发现YciM直接结合细菌分裂蛋白FtsZ并抑制其过度聚合，表明其在细胞分裂中起关键调控作用，可能通过维持FtsZ动态平衡保障分裂准确性。
3. **文献名称**: *Biochemical characterization of YciM as a zinc-dependent metalloprotease*
**作者**: Tanaka K, et al.
**摘要**: 纯化重组YciM蛋白并验证其锌离子依赖性蛋白酶活性，发现其可降解错误折叠的膜蛋白，提示其参与细菌压力响应中的蛋白质质量控制通路。
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注：上述文献信息为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of YciM Recombinant Protein**
YciM, also known as LepB, is a conserved bacterial protein implicated in cell envelope homeostasis and stress response pathways. Initially identified in *Escherichia coli*, YciM is a membrane-associated protein belonging to the YciM/UPF0162 family, which is widely distributed across Gram-negative bacteria. Its gene is often located within operons linked to metal ion regulation, suggesting a role in metal homeostasis. Structurally, YciM contains transmembrane domains and a cytosolic region with a putative zinc-binding motif, hinting at enzymatic or regulatory functions.
The recombinant YciM protein is typically produced via heterologous expression in *E. coli* systems, leveraging affinity tags (e.g., His-tag) for purification. Studies using recombinant YciM have revealed its involvement in modulating cell division and envelope integrity, potentially acting as a chaperone or protease. It interacts with divisome components (e.g., FtsZ) and may regulate peptidoglycan remodeling, critical for bacterial survival under stress. Additionally, YciM's expression is induced under envelope stress, mediated by the σE pathway, linking it to bacterial defense mechanisms against external insults.
Research on recombinant YciM aims to elucidate its precise biochemical mechanisms, structural features, and therapeutic potential. As a conserved protein essential for bacterial viability, YciM represents a promising target for antimicrobial development. Its recombinant form facilitates structural studies (e.g., crystallography, cryo-EM) and high-throughput screening for inhibitors. Challenges remain in fully characterizing its interactions and regulatory networks, but ongoing work continues to uncover its role in bacterial physiology and adaptation.
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