| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ihfA |
| Uniprot No | P0A6X7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-99aa |
| 氨基酸序列 | ALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQVKLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE |
| 预测分子量 | 18.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ihfA重组蛋白的参考文献示例(仅供参考，建议通过学术数据库获取原文)：
1. **标题**: "Cloning, expression, and purification of the ihfA gene product from Escherichia coli"
**作者**: Yang CC, Nash HA
**摘要**: 研究报道了ihfA基因在大肠杆菌中的克隆与表达，通过重组技术获得高纯度蛋白，并证实其与DNA结合的能力。
2. **标题**: "Structural basis for DNA bending by the integration host factor ihfA"
**作者**: Sagi D, et al.
**摘要**: 利用重组ihfA蛋白进行结构分析，揭示其通过诱导DNA弯曲参与基因调控的分子机制。
3. **标题**: "Functional characterization of recombinant IHF subunits in Pseudomonas aeruginosa"
**作者**: Murtin C, et al.
**摘要**: 比较ihfA与ihfB重组蛋白的独立功能，发现ihfA单独存在时仍能部分结合特定DNA序列。
4. **标题**: "Optimizing recombinant ihfA production for biotechnological applications"
**作者**: Gupta P, et al.
**摘要**: 优化表达条件提高ihfA重组蛋白产量，评估其在合成生物学工具开发中的潜力。
**注意**：以上为模拟示例，实际文献请通过PubMed或Google Scholar以关键词“ihfA recombinant protein”检索获取。
**Background of ihfA Recombinant Protein**
The *ihfA* gene encodes the α-subunit of the Integration Host Factor (IHF), a small, heterodimeric DNA-binding protein found in *Escherichia coli* and other bacteria. IHF, composed of IhfA and IhfB subunits, plays a critical role in DNA architecture and gene regulation. It binds to specific sequences, inducing sharp bends in DNA to facilitate processes such as site-specific recombination, transcription initiation, viral integration, and chromosome organization. IHF is particularly vital under stress conditions, aiding bacterial adaptation by modulating global gene expression.
Recombinant IhfA protein is produced through genetic engineering, where the *ihfA* gene is cloned into expression vectors (e.g., plasmids) and expressed in host systems like *E. coli*. Purification techniques such as affinity chromatography yield highly pure, functional IhfA. This recombinant form retains its DNA-binding and bending capabilities, enabling studies on IHF’s structural and regulatory roles.
Research on IhfA recombinant protein has advanced understanding of bacterial nucleoid organization, horizontal gene transfer, and pathogenicity. It also serves as a tool in synthetic biology for designing DNA nanostructures or optimizing genetic circuits. Additionally, targeting IhfA has potential therapeutic applications, as disrupting IHF function could impair bacterial virulence. Overall, IhfA recombinant protein is a key resource for exploring prokaryotic genome dynamics and developing biotechnological or antimicrobial strategies.
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