| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDCD6IP |
| Uniprot No | Q96RI0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-868aa |
| 氨基酸序列 | MATFISVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLR RAAVGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFD KGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNLDNDEGLKIAA KHYQFASGAFLHIKETVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFF LKATRDKMKDAIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPVLAAKHC IMQANAEYHQSILAKQQKKFGEEIARLQHAAELIKTVASRYDEYVNVKDF SDKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLVKSTPVNVPISQK FTDLFEKMVPVSVQQSLAAYNQRKADLVNRSIAQMREATTLANGVLASLN LPAAIEDVSGDTVPQSILTKSRSVIEQGGIQTVDQLIKELPELLQRNREI LDESLRLLDEEEATDNDLRAKFKERWQRTPSNELYKPLRAEGTNFRTVLD KAVQADGQVKECYQSHRDTIVLLCKPEPELNAAIPSANPAKTMQGSEVVN VLKSLLSNLDEVKKEREGLENDLKSVNFDMTSKFLTALAQDGVINEEALS VTELDRVYGGLTTKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLRE EVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKT ERDELLKDLQQSIAREPSAPSIPTPAYQSSPAGGHAPTPPTPAPRTMPPT KPQPPARPPPPVLPANRAPSATAPSPVGAGTAAPAPSQTPGSAPPPQAQG PPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQ QPSYPFPQPPQQSYYPQQ |
| 预测分子量 | 121 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDCD6IP(ALIX)重组蛋白的3篇代表性文献示例(注：文献为示例，实际引用时请核实来源准确性)：
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1. **文献名称**: *Structural basis for ALIX binding to the HIV-1 p6 domain*
**作者**: McCullough J, et al.
**摘要**: 该研究通过晶体学分析揭示了PDCD6IP(ALIX)与HIV-1 p6结构域的相互作用机制，阐明了ALIX在病毒出芽过程中招募ESCRT复合物的分子基础，为重组ALIX蛋白的功能研究提供了结构依据。
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2. **文献名称**: *ALIX regulates tumor extracellular vesicle secretion and metastatic progression*
**作者**: Baietti MF, et al.
**摘要**: 研究利用重组PDCD6IP蛋白进行功能验证，发现ALIX通过调控外泌体分泌促进肿瘤转移，揭示了其在癌症微囊泡生物发生中的关键作用，为靶向ALIX的肿瘤治疗策略提供理论支持。
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3. **文献名称**: *PDCD6IP (ALIX) mediates midbody ring remodeling during cytokinesis*
**作者**: Christ L, et al.
**摘要**: 该文通过重组ALIX蛋白的体外实验，证明其在细胞分裂过程中参与中间体结构的重塑，并协调膜分离与细胞骨架重组，强调了ALIX在细胞周期调控中的多效性功能。
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**提示**：如需实际引用，建议通过PubMed或Google Scholar搜索关键词“PDCD6IP/ALIX recombinant protein”获取最新研究文献。
PDCD6IP, also known as ALIX (ALG-2-interacting protein X), is a multifunctional adaptor protein involved in diverse cellular processes, including membrane remodeling, vesicle trafficking, apoptosis, and viral budding. It plays a central role in the endosomal sorting complex required for transport (ESCRT) pathway, which mediates membrane scission events such as cytokinesis, multivesicular body (MVB) formation, and extracellular vesicle (exosome) release. The protein contains conserved domains, such as the Bro1 domain that binds ESCRT-III components, and a proline-rich region enabling interactions with ALG-2. TSG101. and other partners involved in membrane dynamics.
Recombinant PDCD6IP is engineered to study its structural and functional roles in ESCRT-mediated processes. It is widely used to investigate mechanisms underlying exosome biogenesis, cell membrane repair, and enveloped virus release (e.g., HIV-1). Researchers employ it in binding assays, structural studies, and reconstitution experiments to map interactions with ESCRT machinery or viral proteins like HIV-1 Gag. Its role in apoptosis regulation—via connections to caspases and death receptors—also makes it relevant in cancer and neurodegenerative disease research. Additionally, PDCD6IP's involvement in extracellular vesicle cargo sorting has spurred interest in its potential as a biomarker or therapeutic target. Recombinant forms, often expressed in E. coli or mammalian systems, enable biochemical characterization while preserving native interaction domains critical for its scaffolding functions.
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