| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBP1 |
| Uniprot No | P28147 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200aa |
| 氨基酸序列 | MTDQGLEGSNPVDLSKHPSGIVPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVCTGAKSEDFSKMAARKYARIVQKLGFPAKFKDFKIQNIVGSCDVKFPIRLEGLAYSHAAFSSYEPELFPGLIYRMKVPKIVLLIFVSGKIVITGAKMRDETYKAFENIYPVLSEFRKIQQ |
| 预测分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBP1(TATA结合蛋白1)重组蛋白的3篇参考文献示例(注：部分内容为模拟概括，仅供参考)：
1. **文献名称**：*"Expression and Purification of Recombinant Human TBP1 in Escherichia coli for Functional Studies"*
**作者**：Johnson, R. et al.
**摘要**：该研究报道了通过大肠杆菌表达系统高效表达人源TBP1重组蛋白，并优化了纯化条件。通过凝胶迁移实验(EMSA)验证了重组TBP1与TATA-box DNA序列的特异性结合能力。
2. **文献名称**：*"Structural Analysis of TBP1-DNA Complex by X-ray Crystallography Reveals Binding Mechanism"*
**作者**：Nikolov, D.B. et al.
**摘要**：利用X射线晶体学解析了重组TBP1与TATA-box DNA复合物的三维结构，揭示了TBP1通过β折叠片层嵌入DNA小沟的分子机制，为转录起始复合物组装提供结构基础。
3. **文献名称**：*"Functional Characterization of Recombinant TBP1 in RNA Polymerase II Transcription Initiation"*
**作者**：Hernandez, N. et al.
**摘要**：通过体外转录实验证明重组TBP1是RNA聚合酶II转录起始的核心因子，并发现其与TFIIA、TFIIB等转录因子的协同作用对启动子识别至关重要。
4. **文献名称**：*"Application of Recombinant TBP1 in Studying Gene Regulation in Cancer Models"*
**作者**：Lee, S. et al.
**摘要**：利用重组TBP1蛋白探究其在癌细胞中调控异常基因表达的机制，发现TBP1与特定致癌基因启动子区域的结合增强可能导致转录失调。
(注：以上作者及文献名为示例，实际文献需根据具体研究领域检索，建议通过PubMed或Web of Science以“Recombinant TBP1”或“TATA-binding protein 1”为关键词查询最新论文。)
**Background of TBP1 Recombinant Protein**
TATA-binding protein 1 (TBP1), a core component of the transcription factor IID (TFIID) complex, plays a pivotal role in RNA polymerase II-mediated transcription initiation. It binds to the TATA box, a conserved DNA sequence in eukaryotic gene promoters, facilitating the assembly of the pre-initiation complex (PIC) to regulate gene expression. TBP1 is evolutionarily conserved across eukaryotes and is essential for viability, underscoring its fundamental role in transcriptional regulation.
Recombinant TBP1 is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or eukaryotic expression systems. This allows large-scale production of the protein with high purity and specificity. The recombinant form retains the functional domains of native TBP1. including its DNA-binding saddle-shaped structure and interactions with other transcription factors, making it a valuable tool for *in vitro* studies.
Research applications of recombinant TBP1 include mechanistic studies of transcription initiation, structural analyses (e.g., X-ray crystallography or cryo-EM), and screening for inhibitors or modulators of transcriptional machinery. It is also used to investigate diseases linked to transcriptional dysregulation, such as cancer or neurodegenerative disorders, where TBP1 activity or mutations may contribute to pathogenesis.
The development of recombinant TBP1 has advanced our understanding of gene regulation and provided insights into therapeutic strategies targeting transcriptional anomalies. Its utility in both basic and applied research highlights its importance in molecular biology and biomedicine.
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