| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | psuK |
| Uniprot No | P30235 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313aa |
| 氨基酸序列 | MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN |
| 预测分子量 | 41.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的3篇关于PsuK重组蛋白的参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Heterologous Expression and Functional Characterization of PsuK Protein from Pseudomonas aeruginosa"*
**作者**: Zhang, L. et al.
**摘要**: 本研究在大肠杆菌系统中成功表达了假单胞菌来源的PsuK重组蛋白，通过亲和层析纯化并验证其ATP结合活性。实验表明PsuK可能参与细菌对抗噬菌体感染的防御机制。
2. **文献名称**: *"Structural Insights into PsuK: A Novel Antitermination Factor in Bacteriophage Infection"*
**作者**: Patel, R. & Smith, J.
**摘要**: 通过X射线晶体学解析了PsuK蛋白的三维结构，揭示了其与RNA聚合酶结合的独特结构域，为阐明其在噬菌体感染中调控基因转录终止的分子机制提供依据。
3. **文献名称**: *"Development of a PsuK-based Inhibitor Screening Platform Against Bacterial Pathogens"*
**作者**: Kim, H. et al.
**摘要**: 基于重组PsuK蛋白构建高通量药物筛选模型，鉴定出小分子化合物可阻断PsuK与宿主蛋白互作，为开发新型抗菌疗法奠定基础。
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**提示**：以上内容为模拟生成，建议通过PubMed、Web of Science或Google Scholar检索关键词(如“PsuK recombinant protein”、“PsuK phage antiterminator”)获取真实文献。
The psuK recombinant protein is derived from the psuK gene, originally identified in bacteriophage T4. which encodes a pseudouridine synthase enzyme. Pseudouridine synthases are RNA-modifying enzymes that catalyze the isomerization of specific uridine residues to pseudouridine (Ψ) in RNA molecules, a process critical for RNA stability, structure, and function. The psuK gene product is notable for its role in modifying ribosomal RNA (rRNA) during T4 phage infection, potentially enhancing translational efficiency or evading host defenses. Recombinant psuK protein is typically produced by cloning the gene into expression vectors (e.g., plasmid systems) and expressing it in bacterial hosts like *Escherichia coli*. This allows large-scale purification for structural and functional studies. Researchers utilize recombinant psuK to investigate its catalytic mechanism, substrate specificity, and role in RNA modification, with broader implications for understanding RNA biology and phage-host interactions. Additionally, pseudouridylation has gained attention in biotechnology and therapeutics, particularly in mRNA vaccine design, where Ψ incorporation reduces immunogenicity and improves stability. Studies on psuK may thus contribute to optimizing RNA-based technologies. Structural analyses (e.g., X-ray crystallography or cryo-EM) of the recombinant protein aim to elucidate active-site architecture and guide engineering of novel RNA-modifying tools. Despite its phage origin, psuK’s conserved enzymatic features make it a model for exploring pseudouridine synthase evolution and function across species. Ongoing research focuses on its biotechnological applications and potential as a target for antimicrobial strategies against phage-resistant pathogens.
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