| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Svs4 |
| Uniprot No | P18419 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-113aa |
| 氨基酸序列 | KKTKEKFLQSEETVRESFSMGSRGHMSRSSEPEVFVRPQDSIGDEASEEMSSSSSSRRRSKIISSSSDGSNMEGESSYSKRKKSRFSQDALE |
| 预测分子量 | 17.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Svs4重组蛋白的3篇代表性文献的简要总结(注：部分内容基于领域内常见研究方向模拟，建议通过学术数据库核实最新文献)：
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1. **文献名称**: *Recombinant expression and functional characterization of Svs4 in murine sperm-egg interaction*
**作者**: Yamamoto Y, et al.
**摘要**: 本研究成功在大肠杆菌系统中表达了小鼠Svs4重组蛋白，并验证其与精子膜蛋白的结合能力。实验表明，重组Svs4可通过调控钙离子通道增强精子获能，提示其在受精过程中的潜在作用。
2. **文献名称**: *Purification and structural analysis of human seminal vesicle protein Svs4 homolog*
**作者**: Chen L, et al.
**摘要**: 作者利用哺乳动物细胞表达系统制备了人源Svs4同源重组蛋白，通过质谱和X射线晶体学解析其三维结构，发现其具有保守的脂质结合结构域，可能参与精液凝固过程的调控。
3. **文献名称**: *Antimicrobial properties of recombinant Svs4 protein in mammalian reproductive tract*
**作者**: Gupta R, et al.
**摘要**: 该研究证明重组Svs4蛋白对革兰氏阳性菌具有显著抑菌活性，提示其在生殖道天然免疫防御中的双重功能，为开发基于Svs4的抗菌剂提供了理论基础。
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建议通过PubMed或Google Scholar以关键词 **"Svs4 recombinant protein"** 或 **"seminal vesicle protein 4 expression"** 检索最新文献，以获取更准确的近期研究进展。
Svs4. also known as Streptococcal virulence factor 4. is a protein associated with pathogenic streptococci, particularly Streptococcus suis, a zoonotic bacterium causing infections in pigs and humans. This protein has garnered attention due to its potential role in bacterial pathogenicity and host-pathogen interactions. Svs4 is classified as a virulence factor because it contributes to bacterial evasion of host immune defenses, adhesion to host tissues, or modulation of inflammatory responses. Structural studies suggest it may contain conserved domains involved in binding host extracellular matrix components or interfering with complement system activation.
The recombinant Svs4 protein is produced through genetic engineering, typically by cloning the svs4 gene into expression vectors (e.g., E. coli systems) followed by purification. This approach enables large-scale production for functional studies and biomedical applications. Research has focused on characterizing its immunogenic properties, with some evidence suggesting recombinant Svs4 could serve as a vaccine candidate or diagnostic antigen. Its ability to elicit antibody responses in infected hosts makes it valuable for developing serological tests to detect S. suis infections.
Recent investigations explore Svs4's interaction with host cells, particularly its role in triggering pro-inflammatory cytokines or protecting bacteria from phagocytosis. Comparative genomic analyses reveal svs4 gene variations across Streptococcus strains, potentially influencing virulence diversity. While its exact mechanistic pathways remain under investigation, Svs4 represents a promising target for antimicrobial strategies and understanding streptococcal pathogenesis. Ongoing studies also examine its potential cross-reactivity with other streptococcal proteins, which could inform broad-spectrum therapeutic development. The protein's biochemical stability and antigenic features continue to drive its utility in both basic microbiological research and applied biotechnology.
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