| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | rplB |
| Uniprot No | P60422 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-273aa |
| 氨基酸序列 | AVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK |
| 预测分子量 | 36.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rplB重组蛋白的模拟参考文献示例(仅供参考，具体文献需通过学术数据库核实)：
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1. **文献名称**：*Structural Insights into the Ribosomal L2 Protein through Recombinant Expression in E. coli*
**作者**：Agrawal, R.K., Hermann, T.
**摘要**：本研究报道了通过大肠杆菌重组表达系统高效表达rplB基因编码的核糖体L2蛋白，并利用X射线晶体学解析其三维结构，揭示了L2在核糖体大亚基组装及肽基转移酶活性中的关键作用。
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2. **文献名称**：*Functional Analysis of rplB Mutants in Antibiotic Resistance*
**作者**：Kim, S. et al.
**摘要**：通过定点突变和重组蛋白纯化技术，探究rplB基因突变对细菌核糖体功能的影响，发现L2蛋白的特定结构域与红霉素类抗生素耐药性相关，为靶向药物设计提供依据。
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3. **文献名称**：*Recombinant L2 Protein Facilitates in vitro Ribosome Reconstitution*
**作者**：Müller-Lucks, A. et al.
**摘要**：利用重组表达的L2蛋白，建立体外核糖体重组实验体系，证实L2在核糖体大亚基正确折叠及rRNA结合中的必要性，并验证其与多种核糖体蛋白的相互作用。
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4. **文献名称**：*Biophysical Characterization of Thermostable rplB Recombinant Protein from Thermophiles*
**作者**：Sato, N. et al.
**摘要**：从嗜热菌中克隆rplB基因，通过重组表达获得热稳定性L2蛋白，结合圆二色光谱和微量热法分析其热力学性质，探讨其在高温环境下维持核糖体功能的分子机制。
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**注意**：以上为模拟文献，实际研究中建议通过PubMed、Web of Science等平台以关键词“rplB recombinant protein”“ribosomal protein L2 expression”检索最新成果。
**Background of RplB Recombinant Protein**
The *rplB* gene encodes the 50S ribosomal subunit protein L2. a highly conserved component of the bacterial ribosome. As part of the large ribosomal subunit, L2 plays critical structural and functional roles in translation. It contributes to the formation of the peptidyltransferase center (PTC), the site responsible for catalyzing peptide bond formation during protein synthesis. L2 also interacts with rRNA and other ribosomal proteins, stabilizing the ribosome's architecture and facilitating its assembly. Due to its essential role in translation, *rplB* is a housekeeping gene, widely used as a reference in bacterial gene expression studies.
Recombinant RplB protein is produced through genetic engineering, typically by cloning the *rplB* gene into expression vectors (e.g., *E. coli*) and purifying the protein using affinity tags (e.g., His-tag). This approach allows large-scale production of soluble, functional L2 for biochemical and structural studies. Recombinant technology enables researchers to study L2’s interactions with antibiotics, rRNA, or other ribosomal proteins, providing insights into ribosome function and antibiotic resistance mechanisms.
Interest in RplB also stems from its potential as a therapeutic target. As bacterial ribosomes differ from eukaryotic counterparts, L2 is explored for developing novel antibiotics. Structural studies using recombinant L2 have revealed binding sites for inhibitors, aiding drug design. Additionally, recombinant RplB serves as an antigen in diagnostic tools to detect bacterial infections or in vaccine development.
Overall, RplB recombinant protein is a vital tool for understanding ribosomal biology, bacterial translation mechanisms, and antimicrobial strategies, bridging fundamental research with biotechnological applications.
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