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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Tsku |
| Uniprot No | Q8WUA8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-353 |
| 氨基酸序列 | TRPC FPGCQCEVET FGLFDSFSLT RVDCSGLGPH IMPVPIPLDT AHLDLSSNRL EMVNESVLAG PGYTTLAGLD LSHNLLTSIS PTAFSRLRYL ESLDLSHNGL TALPAESFTS SPLSDVNLSH NQLREVSVSA FTTHSQGRAL HVDLSHNLIH RLVPHPTRAG LPAPTIQSLN LAWNRLHAVP NLRDLPLRYL SLDGNPLAVI GPGAFAGLGG LTHLSLASLQ RLPELAPSGF RELPGLQVLD LSGNPKLNWA GAEVFSGLSS LQELDLSGTN LVPLPEALLL HLPALQSVSV GQDVRCRRLV REGTYPRRPG SSPKVALHCV DTRDSAARGP TIL |
| 预测分子量 | 37.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Tsku重组蛋白的参考文献示例(注：文献信息为示例性内容，建议通过学术数据库检索真实文献)：
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1. **文献名称**: *TSKU Recombinant Protein Regulates Myoblast Differentiation via TGF-β Signaling*
**作者**: Lee H, et al.
**摘要**: 本研究成功表达并纯化了人源Tsku重组蛋白，发现其通过抑制TGF-β信号通路促进成肌细胞分化，为肌肉再生研究提供了潜在分子工具。
2. **文献名称**: *Functional Characterization of TSKU in Extracellular Matrix Remodeling*
**作者**: Zhang Y, et al.
**摘要**: 通过大肠杆菌系统表达Tsku重组蛋白，证实其参与细胞外基质重塑，并调控胶原蛋白沉积，提示其在纤维化疾病中的潜在作用。
3. **文献名称**: *TSKU Recombinant Protein Attenuates Muscle Atrophy in a Murine Model*
**作者**: Smith JL, et al.
**摘要**: 利用哺乳动物细胞表达体系制备Tsku重组蛋白，动物实验表明其可缓解肌肉萎缩模型的症状，机制可能与调节肌肉特异性泛素连接酶相关。
4. **文献名称**: *Crystal Structure and Biochemical Analysis of TSKU Recombinant Protein*
**作者**: Tanaka K, et al.
**摘要**: 解析了Tsku重组蛋白的晶体结构，揭示了其与特定脂质分子的结合位点，为理解其在细胞膜相关信号传导中的功能提供结构基础。
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建议通过PubMed、Web of Science等平台，以“TSKU recombinant protein”、“TSKU gene function”为关键词检索最新研究。
**Background of Tsku Recombinant Protein**
The TSKU (tsukushi) gene encodes a small, secreted protein initially identified as a regulator of embryonic development in vertebrates. It belongs to the leucine-rich repeat superfamily and is characterized by conserved structural motifs, including a signal peptide and a C-terminal hormone-binding domain. TSKU functions as a modulator of signaling pathways, notably interacting with bone morphogenetic proteins (BMPs), transforming growth factor-beta (TGF-β), and fibroblast growth factors (FGFs), playing roles in tissue morphogenesis, cell differentiation, and extracellular matrix organization.
Studies highlight its involvement in ciliogenesis, kidney development, and metabolic regulation. For instance, TSKU knockout models exhibit renal abnormalities and metabolic defects, linking it to ciliopathies and diabetes-related pathways. Its expression is tissue-specific, with high levels observed in the kidney, liver, and reproductive organs.
Recombinant TSKU protein is produced using expression systems like *E. coli* or mammalian cell lines, enabling studies on its biochemical properties and therapeutic potential. As a research tool, it aids in investigating cellular signaling crosstalk, developmental biology, and disease mechanisms. Its therapeutic relevance is emerging in regenerative medicine and metabolic disorder research, particularly for targeting pathways implicated in fibrosis, diabetes, and cystic kidney diseases.
Overall, TSKU recombinant protein serves as a critical reagent for dissecting its multifunctional roles and exploring clinical applications in organ development and metabolic homeostasis.
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