| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNXB |
| Uniprot No | P22105 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673aa |
| 氨基酸序列 | MRLSWSVAQGPFDSFVVQYEDTNGQPQALLVDGDQSKILISGLEPSTPYRFLLYGLHEGKRLGPLSAEGTTGLAPAGQTSEESRPRLSQLSVTDVTTSSLRLNWEAPPGAFDSFLLRFGVPSPSTLEPHPRPLLQRELMVPGTRHSAVLRDLRSGTLYSLTLYGLRGPHKADSIQGTARTLSPVLESPRDLQFSEIRETSAKVNWMPPPSRADSFKVSYQLADGGEPQSVQVDGQARTQKLQGLIPGARYEVTVVSVRGFEESEPLTGFLTTVPDGPTQLRALNLTEGFAVLHWKPPQNPVDTYDVQVTAPGAPPLQAETPGSAVDYPLHDLVLHTNYTATVRGLRGPNLTSPASITFTTGLEAPRDLEAKEVTPRTALLTWTEPPVRPAGYLLSFHTPGGQNQEILLPGGITSHQLLGLFPSTSYNARLQAMWGQSLLPPVSTSFTTGGLRIPFPRDCGEEMQNGAGASRTSTIFLNGNRERPLNVFCDMETDGGGWLVFQRRMDGQTDFWRDWEDYAHGFGNISGEFWLGNEALHSLTQAGDYSMRVDLRAGDEAVFAQYDSFHVDSAAEYYRLHLEGYHGTAGDSMSYHSGSVFSARDRDPNSLLISCAVSYRGAWWYRNCHYANLNGLYGSTVDHQGVSWYHWKGFEFSVPFTEMKLRPRNFRSPAGGG |
| 预测分子量 | 81.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNXB重组蛋白的3篇示例参考文献(注：内容为虚构，仅用于示例格式)：
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1. **文献名称**：*Recombinant TNXB Protein Enhances Collagen Fibrillogenesis in vitro*
**作者**：Smith A, et al.
**摘要**：本研究成功表达并纯化了重组TNXB蛋白，证实其通过调控细胞外基质中胶原纤维的组装，促进胶原网络的稳定性，为TNXB缺陷相关疾病的治疗提供了实验依据。
2. **文献名称**：*Functional Characterization of TNXB in a Mouse Model of Ehlers-Danlos Syndrome*
**作者**：Lee J, et al.
**摘要**：通过重组TNXB蛋白注射治疗TNXB缺陷型小鼠，研究显示其能显著改善皮肤弹性及关节过度活动症状，表明重组TNXB在基因治疗中的潜在应用价值。
3. **文献名称**：*Structural and Biochemical Analysis of Recombinant Tenascin-XB Domains*
**作者**：Garcia-Sanchez C, et al.
**摘要**：利用大肠杆菌系统表达TNXB的功能结构域，解析其与细胞表面受体的相互作用机制，揭示了TNXB在炎症反应和伤口修复中的多重生物学功能。
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如需真实文献，建议在PubMed或Google Scholar检索关键词：
`TNXB recombinant protein`、`Tenascin-XB expression`、`TNXB gene therapy`。
The TNXB gene encodes tenascin-X (TNX), a large extracellular matrix (ECM) glycoprotein belonging to the tenascin family. TNX plays a critical role in regulating collagen fibrillogenesis and maintaining ECM stability, particularly in skin, joints, and blood vessels. It interacts with collagen fibrils and other ECM components, modulating tissue elasticity and mechanical strength. TNX deficiency, caused by mutations in TNXB, is linked to classical-like Ehlers-Danlos syndrome (clEDS), characterized by hypermobility, skin fragility, and tissue laxity. Recombinant TNXB protein refers to TNX produced in vitro using expression systems (e.g., mammalian cells, bacteria) for functional studies or therapeutic exploration. Researchers use recombinant TNX to investigate its molecular interactions, collagen-modulating mechanisms, and potential applications in tissue repair or disease models. Its production often involves optimizing expression conditions to retain functional domains, including fibrinogen-like and epidermal growth factor (EGF)-repeat regions. Challenges include preserving post-translational modifications critical for bioactivity. Current studies focus on leveraging recombinant TNX to develop biomarkers for connective tissue disorders or ECM-targeted therapies.
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