| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R3G |
| Uniprot No | B7ZBB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MEPIGARLSLEAPGPAPFREAPPAEELPAPVVPCVQGGGDGGGASETPSPDAQLGDRPLSPKEEAAPQEQEELLECRRRCRARSFSLPADPILQAAKFLQQQQQQAVALGGEGAEDAQLGPGGCCAKCKKRVQFADTLGLSLASVKHFSEAEEPQVPPAVLSRLRSFPMRAEDLEQLGGLLAAAAVAAPLSAPPSRLRPLFQLPGPSAAAERLQRQRVCLERVQCSTASGAEVKGSGRVLSCPGPRAVTVRYTFTEWRSFLDVPAELQPEPLEPQQPEAPSGASEPGSGDAKKEPGAECFHFSLCLPPGLQPEDEEDADERGVAVHFAVCYRCAQGEYWDNNAGANYTLRYARPADAL |
| 预测分子量 | 45.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R3G重组蛋白的示例参考文献(内容为虚构，仅用于格式示例)：
1. **"PPP1R3G regulates hepatic glycogen synthesis through interaction with AMPK"**
*作者：Zhang L, et al.*
**摘要**：研究揭示了PPP1R3G作为蛋白磷酸酶1(PP1)调节亚基，通过结合AMPK调控肝脏糖原合成的分子机制，重组蛋白实验表明其磷酸化修饰影响PP1的底物特异性。
2. **"Recombinant PPP1R3G expression and its role in insulin signaling"**
*作者：Kim S, et al.*
**摘要**：通过在大肠杆菌中表达并纯化PPP1R3G重组蛋白，发现其直接参与胰岛素介导的糖代谢通路，并增强PP1对糖原合酶的激活作用。
3. **"Structural characterization of PPP1R3G and its binding to PP1"**
*作者：Wang Y, et al.*
**摘要**：利用X射线晶体学解析PPP1R3G重组蛋白的结构，揭示了其保守的PP1结合域，并阐明该相互作用如何调控糖原代谢酶的活性。
4. **"PPP1R3G deficiency exacerbates high-fat diet-induced metabolic disorders"**
*作者：Chen R, et al.*
**摘要**：通过构建PPP1R3G基因敲除小鼠模型，结合重组蛋白功能分析，发现其缺失导致糖原合成障碍，并加重饮食诱导的胰岛素抵抗。
**注**：以上文献为模拟内容，实际研究请通过PubMed或Web of Science等平台检索。
**Background of PPP1R3G Recombinant Protein**
PPP1R3G (Protein Phosphatase 1 Regulatory Subunit 3G) is a member of the PPP1R3 family, which regulates the activity and substrate specificity of Protein Phosphatase 1 (PP1), a serine/threonine phosphatase involved in diverse cellular processes. PPP1R3G acts as a targeting subunit, directing PP1 to specific substrates or subcellular locales to modulate glycogen metabolism. It is particularly implicated in glycogen synthesis and degradation, playing a role in energy homeostasis by influencing enzymes like glycogen synthase (GS) and glycogen phosphorylase.
Structurally, PPP1R3G contains conserved PP1-binding motifs and domains that facilitate interactions with glycogen-associated proteins. Its expression is tissue-specific, with higher levels observed in metabolic organs such as the liver and skeletal muscle. Studies suggest PPP1R3G is regulated by insulin and other metabolic signals, linking it to glucose utilization and storage pathways. Dysregulation of PPP1R3G has been associated with metabolic disorders, including type 2 diabetes and obesity, highlighting its potential as a therapeutic target.
Recombinant PPP1R3G protein is typically produced using bacterial or mammalian expression systems, enabling in vitro studies of its biochemical properties, binding partners, and enzymatic regulation. This tool aids in elucidating mechanisms of glycogen control, insulin resistance, and cellular stress responses. Its applications extend to drug screening, structural studies, and disease modeling, offering insights into metabolic dysfunctions and novel treatment strategies. Research on PPP1R3G continues to uncover its broader roles in cellular signaling beyond glycogen metabolism, emphasizing its importance in maintaining metabolic balance.
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