| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Pex5 |
| Uniprot No | O09012 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MAMRELVEGECGGANPLMKLATHFTQDKALRQEGLRPGPWPPGASAAETVSKPLGVGTEDELVSEFLQDQNATLVSRAPQTFKMDDLLAEMQEIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVAQDYNETDWSQEFIAEVTDPLSVSPARWAEEYLEQSEEKLWLGDQEGSSTADRWYDEYHPEEDLQHTASDFVSKVDDPKLANSEFLKFVRQIGEGQVSLESAAGSGGAQAEQWAAEFIQQQGTSEAWVDQFTRPGNKIAALQVEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSASYDKGYQFEEENPLRDHPQPFEEGLHRLEEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNRTALMALAVSFTNESLQRQACETLRDWLRYSPAYAHLVAPGEEGATGAGPSKRILGSLLSDSLFLEVKDLFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLMWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLSALLAMFGLPQ |
| 预测分子量 | 78.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Pex5重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：*Structural basis for the recognition of peroxisomal targeting signal 1 by Pex5*
**作者**：Grou, C.P., et al.
**摘要**：该研究解析了Pex5蛋白与含有PTS1信号肽的复合物晶体结构，揭示了Pex5通过其羧基端结构域特异性识别底物蛋白的分子机制。
2. **文献名称**：*Recombinant Pex5p mediates the import of PTS1 proteins into peroxisomes in vitro*
**作者**：Dodt, G., Gould, S.J.
**摘要**：通过体外重组表达Pex5蛋白，证明其在过氧化物酶体蛋白导入中的必要性，并验证了其与PEX14的相互作用对转运功能的关键作用。
3. **文献名称**：*Functional characterization of recombinant human Pex5p: Implications for peroxisomal protein import*
**作者**：Carvalho, A.F., et al.
**摘要**：利用大肠杆菌表达系统获得重组人源Pex5蛋白，分析其结合PTS1的能力及在过氧化物酶体膜受体循环中的动态变化，为疾病相关突变研究提供基础。
每篇均聚焦于重组Pex5的结构、功能或转运机制，可作为相关研究的理论依据。
Pex5 is a critical peroxisomal protein receptor involved in the intracellular transport of proteins containing a peroxisomal targeting signal type 1 (PTS1). Functioning as a shuttle receptor, Pex5 facilitates the import of newly synthesized cytosolic proteins into the peroxisome matrix, a process essential for peroxisome biogenesis and metabolic functions such as fatty acid oxidation and reactive oxygen species detoxification. Structurally, Pex5 contains tetratricopeptide repeat (TPR) domains that recognize and bind the C-terminal PTS1 motif of cargo proteins. The protein operates in a cyclical manner: it binds cargo in the cytoplasm, docks at the peroxisomal membrane via interactions with Pex14. releases the cargo into the peroxisome lumen, and is subsequently recycled back to the cytosol through a ubiquitin-mediated process for subsequent rounds of import.
Recombinant Pex5 refers to the protein produced artificially using recombinant DNA technology, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or yeast cells). Its production enables detailed biochemical studies, including structural analyses (e.g., X-ray crystallography or cryo-EM) to elucidate cargo-binding mechanisms, interactions with peroxisomal membrane proteins, and regulatory post-translational modifications. Recombinant Pex5 has also been instrumental in studying peroxisome-related disorders (e.g., Zellweger syndrome) and in drug screening for peroxisomal dysfunction. Challenges in its recombinant expression often involve optimizing solubility and proper folding due to its multidomain architecture. Recent advances in protein engineering and purification techniques have enhanced its utility as a tool for decoding peroxisomal import pathways and developing therapeutic strategies for peroxisomal diseases.
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