| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLRA1 |
| Uniprot No | P23415 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-457aa |
| 氨基酸序列 | MYSFNTLRLYLWETIVFFSLAASKEAEAARSAPKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKSPMLNLFQEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ |
| 预测分子量 | 52.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLRA1重组蛋白的3篇代表性文献摘要的简要整理(文献为假设性示例，基于真实研究方向)：
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1. **标题**：*Expression and functional characterization of recombinant human glycine receptor α1 subunit in HEK293 cells*
**作者**：Lynch, J.W., et al.
**摘要**：研究利用HEK293细胞系统重组表达人源GLRA1蛋白，结合电生理记录分析其离子通道特性，证实重组受体对甘氨酸及拮抗剂(如士的宁)的响应与天然受体一致，为体外药理学研究提供模型。
2. **标题**：*Crystal structure of the human glycine receptor α1 subunit ligand-binding domain*
**作者**：Huang, X., et al.
**摘要**：通过X射线晶体学解析GLRA1胞外结构域与甘氨酸结合的复合物结构，揭示配体结合的关键氨基酸残基及构象变化机制，为靶向药物设计提供结构基础。
3. **标题**：*A disease-associated mutation in GLRA1 causes impaired chloride currents in recombinant receptors*
**作者**：Carta, E., et al.
**摘要**：针对遗传性过度惊跳症患者发现的GLRA1突变(如R271Q)，在重组受体中验证其功能影响，发现突变导致氯离子电流显著降低，阐明致病机制与受体脱敏速率异常相关。
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**说明**：以上文献为示例，实际研究中可参考PubMed等数据库以真实文献为准，关键词包括“GLRA1 recombinant”“glycine receptor expression”“GLRA1 mutation”等。
The glycine receptor alpha 1 (GLRA1) is a critical subunit of the inhibitory glycine receptor (GlyR), a ligand-gated ion channel that mediates synaptic inhibition in the central nervous system. GlyRs are pentameric proteins primarily composed of α and β subunits, with GLRA1 being the predominant α isoform in adult spinal cord and brainstem. These receptors facilitate chloride ion influx upon glycine binding, hyperpolarizing neurons and dampening excitatory signaling. Dysfunction of GLRA1 is linked to hyperekplexia (startle disease), a rare neurological disorder characterized by excessive startle reflexes and muscle stiffness, often caused by missense or nonsense mutations in the GLRA1 gene.
Recombinant GLRA1 protein refers to artificially expressed versions of this subunit, typically produced in heterologous systems like HEK293 or CHO cells for structural and functional studies. Researchers use these proteins to investigate receptor assembly, ligand-binding mechanisms, and channel gating properties. The recombinant approach enables precise manipulation, such as introducing disease-associated mutations to study their pathophysiological effects or testing potential therapeutic compounds.
Structural studies of purified GLRA1. often combined with β subunits, have advanced through cryo-EM and X-ray crystallography, revealing details about glycine-binding pockets, ion permeation pathways, and allosteric modulation sites. These insights guide drug discovery efforts targeting GlyRs for neurological conditions. Additionally, recombinant GLRA1 serves as an antigen for antibody development in diagnostic applications. Its study remains vital for understanding inhibitory neurotransmission and developing treatments for glycinergic pathway disorders.
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