| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | sucB |
| Uniprot No | P0AFG7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-405aa |
| 氨基酸序列 | SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLDV |
| 预测分子量 | 49.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于sucB重组蛋白的假设性参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**: *Cloning and expression of the sucB gene encoding dihydrolipoamide succinyltransferase in Escherichia coli*
**作者**: Zhang L, et al.
**摘要**: 研究报道了通过PCR扩增大肠杆菌sucB基因，构建重组质粒并在E. coli BL21中诱导表达。通过Ni-NTA层析纯化获得可溶性重组蛋白，并验证其琥珀酸脱氢酶复合体中的酶活性。
2. **标题**: *Structural analysis of recombinant SucB protein from Mycobacterium tuberculosis*
**作者**: Patel R, et al.
**摘要**: 利用X射线晶体学解析结核分枝杆菌SucB重组蛋白的3D结构，揭示其与底物结合的关键氨基酸残基，为针对结核病代谢途径的药物设计提供依据。
3. **标题**: *Functional characterization of sucB in Pseudomonas aeruginosa biofilm formation*
**作者**: Kim H, et al.
**摘要**: 通过敲除和回补实验证明，重组表达的SucB蛋白在铜绿假单胞菌生物膜形成中起调控作用，其缺失导致三羧酸循环中断及毒力下降。
4. **标题**: *SucB as a potential vaccine candidate: Immunogenicity assessment in murine models*
**作者**: Gonzalez S, et al.
**摘要**: 评估重组SucB蛋白在小鼠模型中的免疫原性，结果显示其可诱导Th1型免疫应答，提示其在抗细菌感染疫苗开发中的潜力。
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注：以上内容为模拟生成，实际文献需通过学术数据库检索确认。
**Background of SucB Recombinant Protein**
The *sucB* gene encodes dihydrolipoamide succinyltransferase (E2), a critical component of the α-ketoglutarate dehydrogenase complex (KGDHC) in the tricarboxylic acid (TCA) cycle. This mitochondrial multienzyme complex catalyzes the oxidative decarboxylation of α-ketoglutarate to succinyl-CoA, a key step in cellular energy production. In *Escherichia coli*, SucB functions as part of the 2-oxoacid dehydrogenase family, linking carbohydrate metabolism to ATP generation. Its structural and functional conservation across prokaryotes and eukaryotes makes it a valuable model for studying metabolic regulation and enzyme mechanisms.
Recombinant SucB protein is produced via heterologous expression systems, typically using *E. coli* or yeast as hosts. The gene is cloned into expression vectors, allowing large-scale production of the purified protein for biochemical and structural studies. Researchers often employ affinity tags (e.g., His-tags) to facilitate purification and detection.
SucB has garnered interest in both basic and applied research. Structurally, studies focus on its catalytic domains and lipoyl-binding sites to elucidate substrate specificity and interactions within the KGDHC. Dysregulation of KGDHC is linked to neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s), positioning SucB as a potential therapeutic target. In infectious disease research, *sucB* homologs in pathogens like *Mycobacterium tuberculosis* are explored for antimicrobial drug development. Additionally, recombinant SucB serves as an antigen in diagnostic assays for bacterial infections.
Overall, SucB recombinant protein bridges fundamental biochemistry with translational applications, offering insights into metabolic diseases, microbial pathogenesis, and biotechnological innovation.
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