| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNT1 |
| Uniprot No | O60563 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 86.7 kDa |
| 氨基酸序列 | MEGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTIDHPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCIHLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNRLKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERPFLEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSVTKSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQTSNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLNFSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISSRSGNTDKPRPPPLPSEPPPPLPPLPK |
| 预测分子量 | 86.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCNT1重组蛋白的3篇代表性文献(示例为模拟内容，实际文献需根据具体数据库查询)：
1. **《Functional characterization of recombinant human Cyclin T1 protein》**
- **作者**: Smith A, et al.
- **摘要**: 本研究成功在大肠杆菌中表达并纯化重组人源CCNT1蛋白，验证其与CDK9形成P-TEFb复合物的能力，并证明其在RNA聚合酶II转录延伸中的调控作用。
2. **《Structural insights into Cyclin T1 interaction with HIV-1 Tat》**
- **作者**: Lee JH, Kim S.
- **摘要**: 通过重组CCNT1蛋白的晶体结构分析，揭示了其与HIV-1 Tat蛋白结合的关键结构域，为抗病毒药物设计提供了靶点信息。
3. **《Recombinant CCNT1/CDK9 complex enhances in vitro transcription assays》**
- **作者**: Garcia-Rodriguez C, et al.
- **摘要**: 利用昆虫细胞系统共表达并纯化CCNT1/CDK9重组复合物，证明其在体外转录实验中对mRNA合成效率的显著提升，为基因表达研究提供工具。
注：以上内容为示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如"CCNT1 recombinant protein"或"Cyclin T1 expression")获取。
**Background of CCNT1 Recombinant Protein**
CCNT1 (Cyclin T1) is a regulatory protein belonging to the cyclin family, which plays a critical role in controlling cell cycle progression and transcriptional regulation. It is a key component of the positive transcription elongation factor b (P-TEFb) complex, where it binds to cyclin-dependent kinase 9 (CDK9). This complex is essential for the transition from transcription initiation to elongation by phosphorylating the C-terminal domain (CTD) of RNA polymerase II (Pol II), enabling mRNA synthesis.
CCNT1 is particularly notable for its interaction with the HIV-1 Tat protein, which hijacks the P-TEFb complex to enhance viral transcription. This interaction makes CCNT1 a focal point in studies targeting HIV replication. Structurally, CCNT1 contains conserved cyclin domains that mediate binding to CDK9 and other partners, while its unique N-terminal region facilitates interactions with specific transcription factors or viral proteins.
Recombinant CCNT1 protein is produced using expression systems like *E. coli* or mammalian cells, ensuring high purity and activity for experimental use. It serves as a vital tool in studying transcriptional mechanisms, HIV pathogenesis, and CDK9-related signaling. Researchers employ it in *in vitro* kinase assays, protein interaction studies, and drug screening to identify inhibitors of the CDK9/CCNT1 axis for therapeutic applications in cancer, viral infections, and inflammatory diseases. Dysregulation of CCNT1 has been linked to cancers and cardiovascular disorders, further underscoring its biomedical relevance. Overall, CCNT1 recombinant protein is indispensable for dissecting transcriptional control and developing targeted therapies.
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