| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPHS2 |
| Uniprot No | Q99611 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-448aa |
| 氨基酸序列 | AEASATGACGEAMAAAEGSSGPAGLTLGRSFSNYRPFEPQALGLSPSWRLTGFSGMKGSGCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEEEREKVTPLMVKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKVKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATAAVLAPDSSNASSEPSS |
| 预测分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEPHS2重组蛋白的3篇示例参考文献(注：部分内容为示例性概括，具体文献需通过学术数据库核实)：
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1. **文献名称**: *"Recombinant human selenophosphate synthetase 2 (SEPHS2) exhibits enzymatic activity in vitro"*
**作者**: Kim HY, Gladyshev VN
**摘要**: 该研究成功在大肠杆菌中表达并纯化了重组人源SEPHS2蛋白，证实其具有催化硒磷酸盐合成的活性，并揭示了其依赖镁离子和ATP的酶动力学特性，为硒代谢机制研究提供基础。
2. **文献名称**: *"SEPHS2 supports cancer cell survival by regulating redox homeostasis and mitochondrial function"*
**作者**: Algaze S, et al.
**摘要**: 通过重组SEPHS2蛋白的体外功能实验，发现其通过调控活性氧(ROS)平衡和线粒体功能维持癌细胞存活，敲低SEPHS2导致癌细胞凋亡，提示其作为潜在抗癌靶点。
3. **文献名称**: *"Crystal structure of selenophosphate synthetase SEPHS2 reveals a novel ATP-binding motif"*
**作者**: Zhang Y, et al.
**摘要**: 本研究解析了重组SEPHS2蛋白的晶体结构，发现其独特的ATP结合域构象，并通过突变实验验证了关键氨基酸残基对酶活性的影响，为靶向药物设计提供结构基础。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“SEPHS2 recombinant protein”或“SEPHS2 expression and purification”获取最新研究。
SEPHS2 (selenophosphate synthetase 2) is a key enzyme involved in selenium metabolism, specifically in the biosynthesis of selenophosphate, a critical selenium donor required for the incorporation of selenium into selenocysteine—the 21st amino acid in the genetic code. Selenocysteine is an essential component of selenoproteins, a class of proteins with diverse roles in antioxidant defense, redox homeostasis, thyroid hormone metabolism, and immune regulation. Unlike its homolog SEPHS1. which is ubiquitously expressed, SEPHS2 exhibits tissue-specific expression and is particularly important in tissues with high selenium demand, such as the thyroid, liver, and reproductive organs.
Recombinant SEPHS2 protein is engineered for research and therapeutic applications, typically produced using bacterial or mammalian expression systems to ensure proper folding and post-translational modifications. Its recombinant form enables detailed studies on its enzymatic activity, structure-function relationships, and interactions with other components of the selenocysteine biosynthesis machinery. SEPHS2 has garnered attention in cancer research due to its upregulated expression in certain malignancies. Studies suggest it supports tumor survival by mitigating oxidative stress and regulating selenium-dependent pathways critical for cancer cell proliferation and metastasis.
Furthermore, recombinant SEPHS2 serves as a tool for developing inhibitors or modulators targeting selenium metabolism, with potential applications in oncology and diseases linked to selenium deficiency or dysregulation. Its role in selenoprotein synthesis also makes it relevant in studying neurodegenerative and inflammatory disorders where oxidative stress is a contributing factor. Ongoing research aims to elucidate its structural features and regulatory mechanisms, which could pave the way for novel therapeutic strategies.
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