| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HELQ |
| Uniprot No | Q8TDG4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 350-726aa |
| 氨基酸序列 | QERKNLIYSLPTSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKGRFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMTLAKILYTSKTTQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSKAENGMTFSRLLNYKYSDTLKKMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKFLSKEYLKHKEKEKCEVIKNLKNIGNGNLCPVLKRTIPFGVAYHHSGLTSDERKLLEEAYSTGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQYKQMIGRAGRAGIDTIGESILILQEK |
| 预测分子量 | 49.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HELQ重组蛋白的示例参考文献(内容为虚构示例，仅供参考研究方向)：
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1. **文献名称**: *HELQ is a DNA helicase promoting recombination repair by disrupting RAD51 filaments*
**作者**: Adelman, C.A., et al.
**摘要**: 本研究纯化了重组HELQ蛋白，发现其具有ATP依赖的解旋酶活性，可通过破坏RAD51-ssDNA丝状结构促进同源重组修复。实验表明HELQ缺陷导致细胞对DNA交联剂敏感性增加。
2. **文献名称**: *Recombinant HELQ synergizes with BRCA1 in replication fork restart*
**作者**: Takata, M., et al.
**摘要**: 通过体外重建实验，证明重组HELQ蛋白与BRCA1协同作用，在停滞的复制叉处促进DNA复制的重启，并维持基因组稳定性。HELQ的ATP酶活性是其功能的关键。
3. **文献名称**: *Structural insights into HELQ-mediated DNA repair by cryo-EM*
**作者**: Schimmel, J., et al.
**摘要**: 利用冷冻电镜解析重组HELQ蛋白的复合体结构，揭示其与单链DNA结合域及RAD51相互作用的分子机制，为靶向HELQ的癌症治疗提供结构基础。
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**注意**：以上文献为示例，实际研究中建议通过PubMed或Web of Science检索关键词(如“HELQ recombinant protein”“HELQ DNA repair”)获取真实文献。
**Background of HELQ Recombinant Protein**
HELQ (Helicase POLQ-like) is a DNA repair-associated helicase crucial for maintaining genome stability, particularly during replication stress. It belongs to the superfamily 2 (SF2) helicases and plays a role in homologous recombination (HR), a critical pathway for error-free repair of DNA double-strand breaks (DSBs) and stalled replication forks. HELQ interacts with other HR proteins, such as RAD51 and BRCA1/2. to facilitate replication fork restart and prevent genomic rearrangements. Its helicase activity enables unwinding of DNA secondary structures, while its inherent ATPase activity provides energy for these processes.
Studies highlight HELQ's involvement in resolving toxic recombination intermediates and suppressing replication-associated genome instability. Deficiencies in HELQ are linked to increased sensitivity to DNA-damaging agents (e.g., PARP inhibitors) and may contribute to cancer predisposition. Notably, HELQ-deficient cells exhibit synthetic lethality with defects in alternative repair pathways, suggesting its potential as a therapeutic target.
Recombinant HELQ protein is produced via heterologous expression systems (e.g., *E. coli* or insect cells) to study its biochemical properties, structural interactions, and mechanisms in DNA repair. Purified HELQ enables *in vitro* assays analyzing helicase activity, DNA binding, and protein interaction networks. It also aids in drug discovery for cancer therapies targeting DNA repair vulnerabilities.
Research on HELQ recombinant protein enhances understanding of genome maintenance mechanisms and may inform strategies to exploit DNA repair deficiencies in diseases like cancer. Its role in balancing error-free and error-prone repair pathways underscores its significance in both basic biology and translational medicine.
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