| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | S1pr2 |
| Uniprot No | P52592 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-352aa |
| 氨基酸序列 | MGGLYSEYLNPEKVLEHYNYTKETLDMQETTSRKVASAFIIILCCAIVVENLLVLIAVARNSKFHSAMYLFLGNLAASDLLAGVAFVANTLLSGHVTLSLTPVQWFAREGSAFITLSASVFSLLAIAIERQVALAKVKLYGSDKSCRMLMLIGASWLISLILGGLPILGWNCLNQLEACSTVLPLYAKHYVLCVVTIFSVILLAIVALYVRIYFVVRSSHADVAGPQTLALLKTVTIVLGVFIICWLPAFSILLLDSTCPVRACPVLYKAHYFFAFATLNSLLNPVIYTWRSRDLRREVLRPLQCWRRGKGVTGRRGGNPGHRLLPLRSSSSLERGMHMPTSPTFLEGNTVV |
| 预测分子量 | 44.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于S1PR2重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：Structural basis of S1PR2 receptor activation and signaling
**作者**：Smith A, et al.
**摘要**：通过冷冻电镜解析S1PR2重组蛋白与配体结合的三维结构，揭示其激活后偶联下游G蛋白的构象变化机制，为靶向S1PR2的药物设计提供结构基础。
2. **文献名称**：S1PR2 regulates endothelial cell migration through Rho/ROCK pathway
**作者**：Chen L, et al.
**摘要**：利用重组S1PR2蛋白研究其在血管内皮细胞迁移中的作用，证实S1PR2激活后通过RhoA/ROCK信号通路调控细胞骨架重组，影响血管生成和肿瘤微环境。
3. **文献名称**：Targeting S1PR2 with recombinant protein attenuates liver fibrosis
**作者**：Wang Y, et al.
**摘要**：开发S1PR2重组拮抗蛋白并用于小鼠肝纤维化模型，证明其通过抑制肝星状细胞活化和TGF-β信号通路显著减少胶原沉积，提示其作为抗纤维化治疗剂的潜力。
**备注**：以上文献信息为虚拟示例，实际研究中建议通过PubMed(PMID编号检索)或Web of Science平台获取最新文献。可尝试搜索关键词："S1PR2 recombinant protein structure/function/therapeutic"。
**Background of S1PR2 Recombinant Protein**
Sphingosine-1-phosphate receptor 2 (S1PR2), a member of the G protein-coupled receptor (GPCR) family, plays a critical role in mediating cellular responses to sphingosine-1-phosphate (S1P), a bioactive lipid involved in diverse physiological and pathological processes. S1PR2 is ubiquitously expressed, with notable presence in the cardiovascular, immune, and nervous systems. It regulates cell migration, proliferation, angiogenesis, and immune cell trafficking by coupling with intracellular G proteins (Gi, Gq, G12/13), thereby modulating downstream signaling pathways such as MAPK, PI3K/Akt, and Rho GTPases. Dysregulation of S1PR2 is linked to diseases like cancer, fibrosis, atherosclerosis, and neuroinflammatory disorders.
Recombinant S1PR2 protein is engineered to facilitate mechanistic studies and drug discovery. Typically produced in mammalian expression systems (e.g., HEK293 or CHO cells), it retains post-translational modifications essential for ligand binding and receptor activation. The protein often includes tags (e.g., His or Fc) for purification and detection. Structural studies using recombinant S1PR2 have elucidated its ligand-binding pocket and conformational dynamics, aiding in the design of selective agonists/antagonists.
In research, S1PR2 recombinant protein serves as a tool to study receptor-ligand interactions, signaling cascades, and cross-talk with other S1P receptors (S1PR1. 3-5). It also supports high-throughput screening for therapeutics targeting S1PR2-associated diseases. Recent studies highlight its role in tumor microenvironment modulation, vascular permeability, and fibrosis progression, underscoring its therapeutic potential.
Overall, S1PR2 recombinant protein is a vital resource for advancing understanding of S1P biology and developing precision therapies for conditions influenced by S1PR2 dysfunction.
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