| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Cmklr2 |
| Uniprot No | P46090 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-353aa |
| 氨基酸序列 | MEVSREMLFEELDNYSYALEYYSQEPDAEENVYPGIVHWISLLLYALAFVLGIPGNAIVIWFMGFKWKKTVTTLWFLNLAIADFVFVLFLPLYISYVALSFHWPFGRWLCKLNSFIAQLNMFSSVFFLTVISLDRYIHLIHPGLSHPHRTLKNSLLVVLFVWLLASLLGGPTLYFRDTVEVNNRIICYNNFQEYELTLMRHHVLTWVKFLFGYLLPLLTMSSCYLCLIFKTKKQNILISSKHLWMILSVVIAFMVCWTPFHLFSIWELSIHHNSSFQNVLQGGIPLSTGLAFLNSCLNPILYVLISKKFQARFRASVAEVLKRSLWEASCSGTVSEQLRSAETKSLSLLETAQ |
| 预测分子量 | 42.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CMKLR2重组蛋白的3篇代表性文献概览(注：文献信息为模拟示例，具体研究需参考真实数据库)：
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1. **文献名称**：*Recombinant Expression and Functional Characterization of CMKLR2 in Inflammatory Signaling*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了CMKLR2重组蛋白，验证其与配体Chemerin的结合能力，并发现其在巨噬细胞炎症反应中调控NF-κB通路的作用。
2. **文献名称**：*CMKLR2 Structural Insights: Cryo-EM Analysis of a Recombinant Receptor-Ligand Complex*
**作者**：Li X, et al.
**摘要**：通过冷冻电镜解析重组CMKLR2蛋白与Chemerin的复合物结构，揭示了受体激活的分子机制，为靶向药物设计提供结构基础。
3. **文献名称**：*Role of Recombinant CMKLR2 in Adipocyte Metabolism and Insulin Resistance*
**作者**：Wang Y, et al.
**摘要**：利用昆虫细胞系统表达CMKLR2重组蛋白，证明其在脂肪细胞分化及胰岛素敏感性中的双向调节功能，提示其与代谢疾病的潜在关联。
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**备注**：实际研究中，CMKLR2的相关文献可能较为有限(相较于同家族受体CMKLR1)，建议结合关键词“CMKLR2/Chemerin receptor 2”、“recombinant protein expression”及“inflammation/metabolism”进一步检索PubMed或Web of Science获取最新进展。
CMKLR2 (Chemokine-like receptor 2), also known as GPR1. is a class A G protein-coupled receptor (GPCR) that binds chemerin, a multifunctional adipokine involved in immune regulation, adipogenesis, and angiogenesis. Initially identified in 1994. CMKLR2 gained attention for its role in inflammatory responses and metabolic homeostasis. It is expressed in immune cells (e.g., macrophages, dendritic cells), adipose tissue, and the central nervous system, suggesting diverse physiological functions. Structurally, it features seven transmembrane domains typical of GPCRs and activates downstream signaling pathways, including MAPK and PI3K-Akt, upon ligand binding.
Recombinant CMKLR2 protein is engineered using expression systems (e.g., HEK293. insect cells) to produce purified, bioactive forms for functional studies. This protein retains ligand-binding capabilities and signaling activity, enabling researchers to investigate receptor-ligand interactions, signal transduction mechanisms, and its role in diseases. Notably, CMKLR2-chemerin axis dysregulation has been linked to obesity, diabetes, cancer metastasis, and chronic inflammation. For example, in adipose tissue, CMKLR2 modulates adipocyte differentiation and insulin sensitivity, while in cancer, it may promote tumor cell migration.
Research applications of recombinant CMKLR2 include drug screening for metabolic or inflammatory disorders, structural studies to design targeted therapies, and biomarker development. Challenges remain in fully elucidating its tissue-specific signaling and therapeutic potential. Nonetheless, CMKLR2 represents a promising target for modulating immune-metabolic cross-talk, driving ongoing interest in its recombinant form as a tool for mechanistic and translational studies.
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