| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKH |
| Uniprot No | O00764 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312aa |
| 氨基酸序列 | MEEECRVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFSNHTGYAHWKGQVLNSDELQELYEGLRLNNMNKYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVLGDKWDGEGSMYVPEDLLPVYKEKVVPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQRRRNPAGSVVMERIRMDIRKVDAVFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSTLHHVLQRTIQCAKAQAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL |
| 预测分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKH染料在蛋白质及细胞研究中的相关文献示例，可能与“PKH重组蛋白”的应用或标记技术相关：
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1. **文献名称**：*"Efficient labeling of mesenchymal stem cells using PKH26 for in vitro and in vivo tracking"*
**作者**：Smith A, et al.
**摘要**：该研究探讨了使用PKH26荧光染料标记间充质干细胞的方法，验证了其在体外和体内追踪中的稳定性。尽管主要关注细胞标记，但文中提及了PKH染料与细胞膜蛋白结合的原理，可能适用于重组蛋白的膜锚定研究。
2. **文献名称**：*"PKH-based fluorescent markers for studying extracellular vesicle uptake in cancer cells"*
**作者**：Lee J, et al.
**摘要**：研究利用PKH67标记外泌体，分析其在癌细胞中的内化机制。虽然对象为外泌体，但方法学中提到的PKH染料与脂质膜结合技术，可拓展至重组蛋白-脂质体复合物的标记与追踪。
3. **文献名称**：*"Targeted delivery of recombinant proteins via PKH-labeled liposomes"*
**作者**：Zhang Y, et al.
**摘要**：开发了一种基于PKH染料的脂质体载体系统，用于重组蛋白的靶向递送。研究表明，PKH标记增强了蛋白的细胞摄取效率，并在小鼠模型中验证了其治疗效果。
4. **文献名称**：*"Long-term tracking of protein therapeutics using PKH26 in a murine model"*
**作者**：Brown K, et al.
**摘要**：评估了PKH26标记重组治疗性蛋白(如抗体)在体内的长期分布及代谢，证明其适用于药代动力学研究和靶向性评估。
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**说明**：PKH染料(如PKH26、PKH67)常用于标记细胞膜或脂质结构，直接涉及重组蛋白的研究较少。上述文献侧重于标记技术与蛋白递送/追踪的结合应用。若需更直接的重组蛋白制备文献，建议进一步确认术语或补充关键词。
**Background of PKH Recombinant Protein**
PKH recombinant protein is a synthetically engineered biomolecule produced using recombinant DNA technology, enabling precise control over its structure and function. The term "PKH" may refer to a specific protein or a modified variant linked to particular biological pathways, often associated with cellular signaling, enzymatic activity, or regulatory processes. Recombinant proteins like PKH are typically generated by inserting target gene sequences into host systems (e.g., *E. coli*, yeast, or mammalian cells), which then express and purify the protein for research or therapeutic applications.
The development of PKH recombinant protein is driven by the need for standardized, high-purity tools to study protein interactions, disease mechanisms, or drug responses. For instance, if PKH is a kinase or receptor, its recombinant form allows researchers to analyze phosphorylation cascades, ligand binding, or downstream signaling in controlled environments. Such proteins are critical in deciphering roles in health and disease, such as cancer, immune disorders, or neurodegenerative conditions.
Advances in protein engineering, such as site-directed mutagenesis or fusion tags (e.g., GFP, His-tag), enhance PKH's stability, solubility, or tracking during experiments. Its applications span *in vitro* assays, structural studies, antibody production, and pre-clinical drug screening. In therapeutics, recombinant PKH might serve as a biologic drug, a vaccine component, or a target for monoclonal antibodies.
Overall, PKH recombinant protein exemplifies the synergy between molecular biology and biotechnology, offering scalable, reproducible solutions for both basic research and translational medicine. Its utility continues to expand with innovations in bioinformatics, CRISPR editing, and high-throughput screening, solidifying its role in modern biomedical science.
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