| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJD2 |
| Uniprot No | Q9UKL4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321aa |
| 氨基酸序列 | MGEWTILERLLEAAVQQHSTMIGRILLTVVVIFRILIVAIVGETVYDDEQTMFVCNTLQPGCNQACYDRAFPISHIRYWVFQIIMVCTPSLCFITYSVHQSAKQRERRYSTVFLALDRDPPESIGGPGGTGGGGSGGGKREDKKLQNAIVNGVLQNTENTSKETEPDCLEVKELTPHPSGLRTASKSKLRRQEGISRFYIIQVVFRNALEIGFLVGQYFLYGFSVPGLYECNRYPCIKEVECYVSRPTEKTVFLVFMFAVSGICVVLNLAELNHLGWRKIKLAVRGAQAKRKSIYEIRNKDLPRVSVPNFGRTQSSDSAYV |
| 预测分子量 | 36.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GJD2(间隙连接蛋白相关基因)重组蛋白研究的3篇参考文献示例(内容基于领域内常见研究方向概括，具体文献需根据实际检索补充)：
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1. **文献名称**：*Expression and functional characterization of recombinant GJD2 (connexin 36) in mammalian cell lines*
**作者**：Smith A, et al.
**摘要**：本研究通过基因克隆技术在大鼠神经母细胞瘤细胞中表达重组GJD2蛋白(Cx36)，验证其形成功能性间隙连接通道的能力，并证明其在神经元同步电活动中起关键作用。
2. **文献名称**：*Structural analysis of GJD2-mediated gap junctions using cryo-EM*
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析重组GJD2蛋白的分子结构，揭示了其通道孔径和门控机制，为理解其在视网膜神经元间信号传递的分子基础提供依据。
3. **文献名称**：*GJD2 mutations impair electrical synapse formation in zebrafish models*
**作者**：Wang Y, et al.
**摘要**：利用斑马鱼模型研究GJD2基因突变对重组蛋白功能的影响，发现突变导致视网膜神经元间电突触减少，可能与先天性视力障碍相关。
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**注**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“GJD2 recombinant protein”“connexin 36 expression”检索获取。建议结合具体研究方向补充完整引用信息(年份、期刊、DOI等)。
**Background of GJD2 Recombinant Protein**
GJD2 (gap junction protein delta 2), also known as connexin 36 (Cx36), is a member of the connexin family that forms gap junctions—specialized intercellular channels facilitating direct electrical and metabolic communication between cells. Predominantly expressed in neurons and pancreatic β-cells, GJD2 plays a critical role in synchronizing neuronal activity and regulating insulin secretion. Its involvement in neural circuits, particularly in the retina and brain, underscores its importance in visual signaling, circadian rhythms, and cognitive functions. Dysregulation of GJD2 has been linked to neurological disorders (e.g., epilepsy) and metabolic diseases like diabetes.
Recombinant GJD2 protein is engineered using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to produce purified, functional forms of the protein for research. This involves cloning the GJD2 gene into expression vectors, followed by protein extraction and purification via affinity chromatography. The recombinant protein retains structural and functional properties, enabling studies on gap junction assembly, channel gating, and intercellular communication mechanisms.
Researchers utilize GJD2 recombinant protein to investigate its role in cellular networks, screen modulators of gap junction activity, and develop therapeutic strategies targeting connexin-related pathologies. In neuroscience, it aids in modeling synaptic plasticity and network synchronization. In diabetes research, it helps elucidate β-cell coupling mechanisms critical for insulin release.
Overall, GJD2 recombinant protein serves as a vital tool for dissecting connexin biology and bridging gaps in understanding diseases associated with impaired intercellular communication. Its applications span basic research, drug discovery, and translational medicine.
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