| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC23A2 |
| Uniprot No | Q9UGH3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-650aa |
| 氨基酸序列 | MMGIGKNTTSKSMEAGSSTEGKYEDEAKHPAFFTLPVVINGGATSSGEQDNEDTELMAIYTTENGIAEKSSLAETLDSTGSLDPQRSDMIYTIEDVPPWYLCIFLGLQHYLTCFSGTIAVPFLLADAMCVGYDQWATSQLIGTIFFCVGITTLLQTTFGCRLPLFQASAFAFLAPARAILSLDKWKCNTTDVSVANGTAELLHTEHIWYPRIREIQGAIIMSSLIEVVIGLLGLPGALLKYIGPLTITPTVALIGLSGFQAAGERAGKHWGIAMLTIFLVLLFSQYARNVKFPLPIYKSKKGWTAYKLQLFKMFPIILAILVSWLLCFIFTVTDVFPPDSTKYGFYARTDARQGVLLVAPWFKVPYPFQWGLPTVSAAGVIGMLSAVVASIIESIGDYYACARLSCAPPPPIHAINRGIFVEGLSCVLDGIFGTGNGSTSSSPNIGVLGITKVGSRRVIQCGAALMLALGMIGKFSALFASLPDPVLGALFCTLFGMITAVGLSNLQFIDLNSSRNLFVLGFSIFFGLVLPSYLRQNPLVTGITGIDQVLNVLLTTAMFVGGCVAFILDNTIPGTPEERGIRKWKKGVGKGNKSLDGMESYNLPFGMNIIKKYRCFSYLPISPTFVGYTWKGLRKSDNSRSSDEDSQATG |
| 预测分子量 | 71.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC23A2重组蛋白的假设性参考文献示例(请注意，部分文献为虚构，仅供格式参考)：
1. **文献名称**: "Functional Characterization of Recombinant Human SLC23A2/SVCT2 Expressed in Yeast"
**作者**: Lutsenko S, et al.
**摘要**: 本研究利用酵母表达系统成功表达了重组人源SLC23A2蛋白，并验证其抗坏血酸转运活性。通过放射性标记实验证明，该重组蛋白对L-抗坏血酸具有高亲和力(Km≈65 μM)，且依赖钠离子梯度驱动转运。
2. **文献名称**: "Cryo-EM Structure of SLC23A2 Reveals Substrate Recognition Mechanism"
**作者**: Subramanian VS, et al.
**摘要**: 通过冷冻电镜解析了SLC23A2重组蛋白的3.2 Å分辨率结构，揭示了跨膜结构域中抗坏血酸结合口袋的关键氨基酸残基(如Tyr423和Glu490)，为理解其底物特异性提供结构基础。
3. **文献名称**: "SLC23A2 Overexpression Enhances Chemosensitivity in Colorectal Cancer Cells"
**作者**: Mendoza-Hill D, et al.
**摘要**: 在结直肠癌细胞系中过表达重组SLC23A2蛋白，发现其通过增加细胞内维生素C水平增强奥沙利铂的细胞毒性，提示该蛋白可能作为化疗增敏靶点。
4. **文献名称**: "Development of a High-Throughput Assay for SLC23A2 Transport Activity Using Recombinant Protein"
**作者**: Souza RF, et al.
**摘要**: 基于HEK293细胞表达的重组SLC23A2蛋白，建立荧光探针(DAA)动态检测方法，用于筛选调控其转运活性的小分子化合物，发现两种新型变构抑制剂(IC50<1 μM)。
**注意**：以上文献为模拟示例，实际研究中请通过PubMed或Web of Science等平台检索真实发表的论文。
**Background of SLC23A2 Recombinant Protein**
The SLC23A2 gene encodes solute carrier family 23 member 2 (SVCT2), a sodium-dependent vitamin C transporter critical for cellular uptake of ascorbic acid (vitamin C), an essential antioxidant and cofactor in numerous enzymatic reactions. SVCT2 is primarily expressed in metabolically active and stress-prone tissues, including the brain, eyes, adrenal glands, and placenta, where it maintains intracellular vitamin C homeostasis. Unlike its isoform SVCT1 (SLC23A1), which localizes to epithelial tissues for dietary absorption, SVCT2 ensures vitamin C delivery to cells incapable of synthesizing it, particularly in humans who lack the gulonolactone oxidase enzyme required for endogenous vitamin C production.
Recombinant SLC23A2 protein is produced using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its structure-function relationships, transport kinetics, and regulatory mechanisms. The protein typically retains key features, such as 12 predicted transmembrane domains and sodium-binding sites, enabling researchers to investigate its role in health and disease. Studies using recombinant SVCT2 have highlighted its importance in neuroprotection, immune function, and collagen synthesis, while dysregulation is linked to oxidative stress-related disorders, neurodegenerative diseases, and cancer progression.
Additionally, recombinant SLC23A2 serves as a tool for drug discovery, aiding in screening compounds that modulate vitamin C transport or address deficiencies in conditions like diabetes or chronic inflammation. Its applications extend to developing therapeutic strategies targeting tissues reliant on SVCT2 for antioxidant defense, such as in age-related macular degeneration or ischemic injuries. Overall, SLC23A2 recombinant protein remains pivotal in elucidating vitamin C biology and its translational implications.
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