| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHCE |
| Uniprot No | P18577 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-417aa |
| 氨基酸序列 | MSSKYPRSVRRCLPLWALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAALGLGFLTSNFRRHSWSSVAFNLFMLALGVQWAILLDGFLSQFPPGKVVITLFSIRLATMSAMSVLISAGAVLGKVNLAQLVVMVLVEVTALGTLRMVISNIFNTDYHMNLRHFYVFAAYFGLTVAWCLPKPLPKGTEDNDQRATIPSLSAMLGALFLWMFWPSVNSPLLRSPIQRKNAMFNTYYALAVSVVTAISGSSLAHPQRKISMTYVHSAVLAGGVAVGTSCHLIPSPWLAMVLGLVAGLISIGGAKCLPVCCNRVLGIHHISVMHSIFSLLGLLGEITYIVLLVLHTVWNGNGMIGFQVLLSIGELSLAIVIALTSGLLTGLLLNLKIWKAPHVAKYFDDQVFWKFPHLAVGF |
| 预测分子量 | 47.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与RHCE重组蛋白相关的文献示例(注：以下内容为模拟概括，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *Expression of recombinant RhCE proteins for antibody detection in transfusion medicine*
**作者**: Smith, J.R., et al.
**摘要**: 研究通过哺乳动物细胞表达系统重组生产RhCE蛋白，验证其在血清学检测中的有效性。实验表明重组蛋白可替代传统红细胞用于抗-Ce抗体筛查，提高检测标准化程度。
2. **文献名称**: *Comparative analysis of native and recombinant RhCE antigens in blood typing*
**作者**: Lee, H., & Chen, Y.
**摘要**: 对比天然红细胞膜RhCE抗原与重组蛋白的结构差异，发现重组蛋白保留关键抗原表位。该技术为稀有血型抗体鉴定提供稳定抗原来源，减少对捐赠红细胞的依赖。
3. **文献名称**: *Engineering recombinant RhD/RhCE chimeric proteins for epitope mapping*
**作者**: Müller, T.H., et al.
**摘要**: 构建RhD/RhCE嵌合重组蛋白，系统定位Rh血型系统关键抗原表位。研究证实重组蛋白技术可精准解析临床疑难抗体特异性，推动精准输血策略发展。
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**说明**：实际研究中可重点检索《Transfusion》《Blood》《Vox Sanguinis》等血液学期刊，关键词包括“recombinant Rh protein”“RhCE expression”“blood group genotyping”。真实文献需通过PubMed、Web of Science等平台验证。
Background of RHCE Recombinant Protein
The RHCE recombinant protein is a critical component in studying the Rh blood group system, one of the most clinically significant blood type classifications in transfusion medicine. The Rh system comprises over 50 antigens, with D (RhD) and CE (RhCE) being the most immunogenic. RHCE genes encode transmembrane proteins that carry Ce and Ee antigens on red blood cells (RBCs), playing essential roles in cell membrane stability and antigen-antibody interactions. Historically, research relied on RBCs from human donors, which posed limitations in supply consistency and biosafety risks.
Recombinant RHCE protein technology emerged to address these challenges. Using genetic engineering, RHCE genes are cloned into expression vectors (e.g., mammalian or insect cell systems) to produce purified RhCE antigens. Mammalian systems like CHO cells are preferred for their ability to perform human-like post-translational modifications, ensuring proper protein folding and antigenic integrity. The process involves codon optimization, transfection, affinity chromatography purification, and quality validation via Western blot or flow cytometry.
This approach offers several advantages: ① Eliminates donor dependency and biohazard risks; ② Provides standardized antigens for serological assays; ③ Enables structural studies of Rh epitopes; ④ Supports diagnostic kit development for antibody detection in pregnancy or transfusion settings. Current applications include antibody identification panels, RBC-mimetic nanoparticles, and research tools for investigating hemolytic disease mechanisms. Ongoing efforts focus on improving expression yields and preserving conformational epitopes critical for clinical accuracy. As a recombinant alternative to native RBC antigens, RHCE proteins enhance the safety and scalability of blood group testing while advancing fundamental research in hematology and immunology.
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