| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK1IP1L |
| Uniprot No | Q8NDC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-245aa |
| 氨基酸序列 | SDEFSLADALPEHSPAKTSAVSNTKPGQPPQGWPGSNPWNNPSAPSSVPSGLPPSATPSTVPFGPAPTGMYPSVPPTGPPPGPPAPFPPSGPSCPPPGGPYPAPTVPGPGPTGPYPTPNMPFPELPRPYGAPTDPAAAGPLGPWGSMSSGPWAPGMGGQYPTPNMPYPSPGPYPAPPPPQAPGAAPPVPWGTVPPGAWGPPAPYPAPTGSYPTPGLYPTPSNPFQVPSGPSGAPPMPGGPHSYH |
| 预测分子量 | 53.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAPK1IP1L重组蛋白的3篇虚构参考文献示例，格式为文献名称、作者及摘要概括：
---
1. **文献名称**: *Expression and Purification of Recombinant MAPK1IP1L in E. coli*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功表达并纯化了重组MAPK1IP1L蛋白，通过优化诱导条件和亲和层析技术获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**: *MAPK1IP1L Modulates MAPK Signaling Pathway in Cellular Stress Response*
**作者**: Lee S, et al.
**摘要**: 研究发现重组MAPK1IP1L通过结合MAPK1(ERK2)调控应激信号通路，在细胞氧化应激模型中抑制MAPK过度激活，提示其潜在抗氧化损伤功能。
3. **文献名称**: *Structural Insights into MAPK1IP1L Interaction with JNK Kinase*
**作者**: Müller R, et al.
**摘要**: 通过X射线晶体学解析重组MAPK1IP1L与JNK1的复合物结构，揭示其通过保守结构域参与MAPK信号调控，为靶向药物设计提供理论依据。
---
注：以上内容为模拟示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of MAPK1IP1L Recombinant Protein**
MAPK1IP1L (Mitogen-Activated Protein Kinase 1 Interacting Protein 1-Like), also known as MBIP, is a regulatory protein involved in modulating the MAPK signaling pathway, a critical cascade governing cellular responses to external stimuli, including proliferation, differentiation, and apoptosis. It shares homology with MAPK1IP1 but exhibits distinct functional roles. MAPK1IP1L interacts with components of the MAPK pathway, particularly ERK1/2 (Extracellular Signal-Regulated Kinase 1/2), influencing their activity and downstream signaling.
The recombinant MAPK1IP1L protein is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity. It typically includes affinity tags (e.g., His or GST tags) to facilitate purification and detection. This recombinant form enables researchers to study MAPK1IP1L’s structural and functional properties in vitro, including its role in scaffolding MAPK components, regulating kinase activity, or mediating cross-talk with other pathways.
Studies suggest MAPK1IP1L may act as a tumor suppressor or oncogenic modulator, depending on cellular context, with implications in cancers, neurodegenerative disorders, and inflammatory diseases. Its recombinant variant is pivotal for elucidating mechanisms of MAPK dysregulation, screening therapeutic agents, and developing diagnostic tools. Further research aims to clarify its tissue-specific functions and potential as a biomarker or drug target, advancing precision medicine strategies.
×
