| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLP2R |
| Uniprot No | GLP2R |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MKLGSSRAGPGRGSAGLLPGVHELPMGIPAPWGTSPLSFHRKCSLWAPGRPFLTLVLLVSIKQVTGSLLEETTRKWAQYKQACLRDLLKEPSGIFCNGTFDQYVCWPHSSPGNVSVPCPSYLPWWSEESSGRAYRHCLAQGTWQTIENATDIWQDDSECSENHSFKQNVDRYALLSTLQLMYTVGYSFSLISLFLALTLLLFLRKLHCTRNYIHMNLFASFILRTLAVLVKDVVFYNSYSKRPDNENGWMSYLSEMSTSCRSVQVLLHYFVGANYLWLLVEGLYLHTLLEPTVLPERRLWPRYLLLGWAFPVLFVVPWGFARAHLENTGCWTTNGNKKIWWIIRGPMMLCVTVNFFIFLKILKLLISKLKAHQMCFRDYKYRLAKSTLVLIPLLGVHEILFSFITDDQVEGFAKLIRLFIQLTLSSFHGFLVALQYGFANGEVKAELRKYWVRFLLARHSGCRACVLGKDFRFLGKCPKKLSEGDGAEKLRKLQPSLNSGRLLHLAMRGLGELGAQPQQDHARWPRGSSLSECSEGDVTMANTMEEILEESEI |
| 预测分子量 | 64.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLP2R重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Structural basis of GLP-1 and GLP-2 receptor binding and activation by glucagon-like peptides"*
**作者**：Zhang Y. et al.
**摘要**：该研究解析了GLP2R与配体结合的冷冻电镜结构，揭示了重组表达的GLP2R与GLP-2肽的结合模式及信号传导机制，为开发靶向GLP2R的代谢疾病药物提供了结构基础。
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2. **文献名称**：*"Functional characterization of recombinant GLP2R variants in intestinal epithelial cells"*
**作者**：Munroe D.G. et al.
**摘要**：通过重组表达人源GLP2R蛋白，研究其在肠道上皮细胞中的功能，发现GLP2R激活可显著促进细胞增殖，并阐明了其下游cAMP/PKA信号通路的关键作用。
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3. **文献名称**：*"Production and purification of bioactive recombinant human GLP2R for therapeutic antibody screening"*
**作者**：Jiang G. et al.
**摘要**：报道了利用哺乳动物表达系统高效制备重组人GLP2R蛋白的方法，优化纯化工艺后获得高活性受体，成功用于筛选治疗短肠综合征的单克隆抗体。
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*注：若需具体文献来源，建议通过PubMed或Sci-Hub输入标题或作者名获取全文。*
The glucagon-like peptide-2 receptor (GLP2R) is a class B G protein-coupled receptor (GPCR) that plays a critical role in regulating intestinal homeostasis, nutrient absorption, and mucosal repair. It is primarily expressed in the gastrointestinal tract, particularly in enteroendocrine cells, enteric neurons, and myofibroblasts. The receptor binds its endogenous ligand, glucagon-like peptide-2 (GLP-2), a 33-amino acid peptide derived from proglucagon. Activation of GLP2R triggers intracellular signaling cascades, predominantly via the Gαs-adenylyl cyclase pathway, leading to enhanced crypt cell proliferation, reduced apoptosis, and improved barrier function. These effects underscore its therapeutic potential in conditions like short bowel syndrome, inflammatory bowel disease, and chemotherapy-induced enteropathy.
Recombinant GLP2R proteins are engineered to study receptor-ligand interactions, signaling mechanisms, and drug discovery. Produced through heterologous expression systems (e.g., mammalian HEK293 or insect cells), recombinant GLP2R retains structural and functional integrity, enabling in vitro characterization. Researchers often fuse affinity tags (e.g., FLAG or His-tag) to facilitate purification and detection. Structural studies using cryo-EM and X-ray crystallography of recombinant GLP2R have provided insights into ligand-binding domains and activation conformations, aiding the design of GLP-2 analogs or small-molecule agonists. Challenges include maintaining receptor stability during purification and ensuring proper post-translational modifications critical for ligand binding. Current applications extend to high-throughput screening for therapeutics targeting metabolic and gastrointestinal disorders, highlighting its translational relevance in precision medicine.
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