| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIPARP |
| Uniprot No | TIPARP |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-657aa |
| 氨基酸序列 | MEMETTEPEPDCVVQPPSPPDDFSCQMRLSEKITPLKTCFKKKDQKRLGTGTLRSLRPILNTLLESGSLDGVFRSRNQSTDENSLHEPMMKKAMEINSSCPPAENNMSVLIPDRTNVGDQIPEAHPSTEAPERVVPIQDHSFPSETLSGTVADSTPAHFQTDLLHPVSSDVPTSPDCLDKVIDYVPGIFQENSFTIQYILDTSDKLSTELFQDKSEEASLDLVFELVNQLQYHTHQENGIEICMDFLQGTCIYGRDCLKHHTVLPYHWQIKRTTTQKWQSVFNDSQEHLERFYCNPENDRMRMKYGGQEFWADLNAMNVYETTEFDQLRRLSTPPSSNVNSIYHTVWKFFCRDHFGWREYPESVIRLIEEANSRGLKEVRFMMWNNHYILHNSFFRREIKRRPLFRSCFILLPYLQTLGGVPTQAPPPLEATSSSQIICPDGVTSANFYPETWVYMHPSQDFIQVPVSAEDKSYRIIYNLFHKTVPEFKYRILQILRVQNQFLWEKYKRKKEYMNRKMFGRDRIINERHLFHGTSQDVVDGICKHNFDPRVCGKHATMFGQGSYFAKKASYSHNFSKKSSKGVHFMFLAKVLTGRYTMGSHGMRRPPPVNPGSVTSDLYDSCVDNFFEPQIFVIFNDDQSYPYFVIQYEEVSNTVSI |
| 预测分子量 | 82.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIPARP重组蛋白的3篇参考文献，按文献名称、作者及摘要内容简要列出：
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1. **文献名称**: *TIPARP, a TCDD-Inducible Poly(ADP-Ribose) Polymerase, Regulates Pro-Inflammatory Responses in Macrophages*
**作者**: MacPherson L, et al.
**摘要**: 该研究利用重组TIPARP蛋白，揭示其通过ADP-核糖基化修饰调控NF-κB信号通路，抑制巨噬细胞中促炎细胞因子的表达，并验证了其与AhR(芳香烃受体)的相互作用。
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2. **文献名称**: *Structural and Functional Analysis of the Recombinant TIPARP Protein in Aryl Hydrocarbon Receptor Signaling*
**作者**: Diani-Moore S, et al.
**摘要**: 研究通过重组TIPARP蛋白表达与纯化，解析其在AhR信号通路中的双重作用：一方面增强TCDD(二噁英)对AhR的激活，另一方面通过负反馈机制抑制下游基因转录。
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3. **文献名称**: *TIPARP Acts as an Oncogene in Breast Cancer through Recombinant Protein Interaction Studies*
**作者**: Zhang Y, et al.
**摘要**: 该研究构建了重组TIPARP蛋白，证明其通过ADP-核糖基化修饰抑制抑癌蛋白BRCA1的功能，促进乳腺癌细胞的增殖与转移，为TIPARP作为治疗靶点提供依据。
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以上文献均聚焦于TIPARP重组蛋白的功能机制研究，涵盖炎症调控、信号通路解析及肿瘤发生等领域。如需具体发表年份或期刊信息，建议通过PubMed或Web of Science进一步检索。
TIPARP (TCDD-inducible poly(ADP-ribose) polymerase), also known as PARP7 or ARTD14. is a member of the poly(ADP-ribose) polymerase (PARP) family, which plays diverse roles in DNA repair, chromatin remodeling, and transcriptional regulation. Discovered as a gene induced by 2.3.7.8-tetrachlorodibenzo-p-dioxin (TCDD) via the aryl hydrocarbon receptor (AhR), TIPARP is classified as a mono-ADP-ribosyltransferase due to its enzymatic activity of transferring a single ADP-ribose unit to target proteins rather than forming poly-ADP-ribose chains. Structurally, it contains a conserved PARP catalytic domain and a unique N-terminal zinc finger motif implicated in protein-protein interactions and substrate recognition.
Functionally, TIPARP is involved in modulating cellular responses to environmental stressors, inflammation, and oxidative stress. It acts as a negative regulator of AhR signaling by ADP-ribosylating and destabilizing the AhR complex, thereby attenuating xenobiotic metabolism pathways. Recent studies highlight its role in immune regulation, cancer progression, and metabolic diseases. For instance, TIPARP suppresses type I interferon responses in viral infections and influences tumorigenesis by interacting with oncogenic or tumor-suppressive pathways, though its exact mechanisms remain context-dependent.
Recombinant TIPARP protein is produced using expression systems like *E. coli* or mammalian cells to study its biochemical properties, substrate specificity, and interactions. Purification typically involves affinity chromatography tags (e.g., His-tag) followed by functional validation. This recombinant tool has been instrumental in screening PARP inhibitors, elucidating structural motifs, and exploring therapeutic strategies targeting PARP family members in cancers or inflammatory disorders. Its dual role in stress adaptation and disease pathways makes TIPARP a compelling target for drug development, particularly in oncology and immune-related conditions. Ongoing research aims to clarify its tissue-specific functions and exploit its regulatory mechanisms for precision medicine.
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