| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNTI |
| Uniprot No | Q9XGM8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-444aa |
| 氨基酸序列 | RLFQTQSQYADRLSSAIESENHCTSQMRGLIDEVSIKQSRIVALEDMKNRQDEELVQLKDLIQTFEKKGIAKLTQGGQMPVAAVVVMACSRADYLERTVKSVLTYQTPVASKYPLFISQDGSDQAVKSKSLSYNQLTYMQHLDFEPVVTERPGELTAYYKIARHYKWALDQLFYKHKFSRVIILEDDMEIAPDFFDYFEAAASLMDRDKTIMAASSWNDNGQKQFVHDPYALYRSDFFPGLGWMLKRSTWDELSPKWPKAYWDDWLRLKENHKGRQFIRPEVCRTYNFGEHGSSLGQFFSQYLEPIKLNDVTVDWKAKDLGYLTEGNYTKYFSGLVRQARPIQGSDLVLKAQNIKDDVRIRYKDQVEFERIAGEFGIFEEWKDGVPRTAYKGVVVFRIQTTRRVFLVGPDSVMQLGIRNS |
| 预测分子量 | 51.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNTI重组蛋白的虚构参考文献示例，基于常见研究方向概括：
1. **文献名称**: *Design and Application of GNTI Recombinant Protein in Non-Viral Gene Delivery Systems*
**作者**: Smith J, et al.
**摘要**: 研究开发了一种基于GNTI重组蛋白的新型基因递送载体，通过体外实验证明其能高效结合DNA并靶向肿瘤细胞，显著提升转染效率且细胞毒性较低。
2. **文献名称**: *Crystal Structure and Functional Characterization of GNTI Recombinant Protein*
**作者**: Lee H, et al.
**摘要**: 利用X射线衍射技术解析了GNTI重组蛋白的三维结构，揭示了其与G蛋白偶联受体激酶(GRK)相互作用的特异性位点，为开发靶向治疗药物提供结构基础。
3. **文献名称**: *High-Yield Production of GNTI Recombinant Protein via E. coli Expression System Optimization*
**作者**: Zhang R, et al.
**摘要**: 通过优化大肠杆菌表达系统的培养条件和诱导策略，实现了GNTI重组蛋白的高效可溶性表达，纯化后蛋白活性验证为后续研究奠定基础。
4. **文献名称**: *GNTI Recombinant Protein Attenuates Inflammation in Murine Models via Modulation of MAPK Signaling*
**作者**: Brown K, et al.
**摘要**: 在炎症小鼠模型中，GNTI重组蛋白通过调控MAPK通路抑制促炎因子释放，表明其在治疗免疫相关疾病中的潜在应用价值。
(注：以上文献为示例性内容，实际研究中请以具体领域文献为准。)
GNTI (G Protein-Coupled Receptor Kinase Interacting Protein 1) recombinant protein is a biologically engineered molecule derived from the human *GIT1* gene, which encodes a multifunctional scaffold protein involved in cellular signaling pathways. Initially identified for its role in regulating G protein-coupled receptor (GPCR) internalization and recycling, GIT1 interacts with various partners, including kinases, GTPases, and cytoskeletal components, to modulate processes like cell migration, synaptic plasticity, and receptor trafficking.
The recombinant form of GNTI is produced using expression systems such as *E. coli* or mammalian cells, enabling large-scale purification for research and therapeutic applications. Its design often includes affinity tags (e.g., His-tag) to facilitate isolation. Structurally, recombinant GNTI retains functional domains critical for binding ARF GTPases, paxillin, and other signaling molecules, making it a valuable tool for studying intracellular communication networks.
In neuroscience, GNTI recombinant protein has been employed to investigate synaptic vesicle dynamics and neurotransmitter release, particularly in disorders like Parkinson’s disease and schizophrenia. In cancer research, it aids in exploring metastasis mechanisms due to its role in cell adhesion and motility. Therapeutic potential is also being explored, such as in modulating GPCR pathways implicated in cardiovascular diseases.
Challenges in its application include maintaining post-translational modifications in heterologous systems and ensuring stability in vivo. Ongoing studies focus on optimizing expression protocols and engineering variants with enhanced specificity. As a versatile reagent, GNTI recombinant protein bridges molecular biology and translational medicine, offering insights into complex signaling cascades and their dysregulation in disease.
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