| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACP3 |
| Uniprot No | P15309 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-386aa |
| 氨基酸序列 | MRAAPLLLARAASLSLGFLFLLFFWLDRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQGTEDSTD |
| 预测分子量 | 44.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACP3重组蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库检索)：
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1. **标题**: *Expression and Purification of Recombinant Human ACP3 in Escherichia coli*
**作者**: Smith J, et al.
**摘要**: 研究报道了利用大肠杆菌表达系统高效生产重组人源ACP3蛋白的方法，并通过亲和层析纯化获得高纯度蛋白，验证了其磷酸酶活性及稳定性。
2. **标题**: *Functional Analysis of Recombinant ACP3 in Tumor Cell Signaling*
**作者**: Lee H, et al.
**摘要**: 通过重组ACP3蛋白的功能实验，揭示其在肿瘤细胞中调控MAPK信号通路的作用，为癌症治疗提供潜在靶点。
3. **标题**: *Structural Insights into Recombinant ACP3 from Mouse: Implications for Substrate Specificity*
**作者**: Zhang Y, et al.
**摘要**: 解析了小鼠重组ACP3的晶体结构，发现其活性口袋的独特构象，解释了其对特定磷酸酯底物的催化机制。
4. **标题**: *Development of a Recombinant ACP3-Based Serological Diagnostic Kit for Metabolic Disorders*
**作者**: Brown K, et al.
**摘要**: 利用重组ACP3蛋白开发新型诊断试剂，成功检测患者血清中ACP3抗体水平，关联代谢异常疾病标志物。
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**注意**：以上为假设性示例，实际文献需通过PubMed、Web of Science等平台以关键词“recombinant ACP3 protein”或“ACP3 expression”检索。推荐结合具体研究领域(如癌症、酶学)进一步筛选。
**Background on ACP3 Recombinant Protein**
ACP3 (Acid Phosphatase 3), also known as prostatic acid phosphatase (PAP), is a lysosomal enzyme encoded by the *ACP3* gene in humans. It belongs to the histidine acid phosphatase family and plays a role in hydrolyzing phosphate esters in an acidic environment. Initially identified for its high expression in the prostate gland, ACP3 has been extensively studied as a clinical biomarker for prostate cancer. However, recent research has revealed its broader physiological relevance, including roles in bone metabolism, immune regulation, and neuronal signaling.
Recombinant ACP3 protein is produced using biotechnological platforms, such as *E. coli* or mammalian cell expression systems, to ensure high purity and activity. Its production involves cloning the *ACP3* gene into expression vectors, followed by protein purification via affinity chromatography. The recombinant form retains enzymatic activity and structural stability, enabling in vitro studies to elucidate its biological functions and interactions.
ACP3’s significance extends beyond oncology. In immunology, it modulates T-cell activation and is linked to autoimmune diseases. In neuroscience, it influences synaptic plasticity, potentially impacting neurodegenerative disorders. Additionally, ACP3 has been explored as a therapeutic target; for example, its immunomodulatory properties underpin the development of PAP-based vaccines for prostate cancer.
Despite progress, challenges remain in understanding isoform-specific functions and regulatory mechanisms. Recombinant ACP3 serves as a critical tool for structural analysis, inhibitor screening, and functional assays. Ongoing research aims to clarify its role in cellular signaling pathways and exploit its therapeutic potential, reinforcing its importance in both basic and applied biomedical sciences.
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