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Mouse Monoclonal BAX Antibody

  • 中文名： BAX抗体
  • 别    名： BCL2L4
货号: IPD31902
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500 - 1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/200-1/400 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesBCL2L4
Entrez GeneID581
clone2G6B2
WB Predicted band size21.2kDa
Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human BAX expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是关于BAX抗体的3篇参考文献示例(内容为虚构，仅供格式参考)：

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1. **文献名称**：*BAX Antibody Specificity in Apoptosis Detection: A Comparative Study*

**作者**：Smith A, et al.

**摘要**：本研究评估了多种商业BAX抗体的特异性，通过Western blot和免疫荧光技术验证其在检测内源性BAX蛋白中的可靠性，发现克隆号6A7在凋亡细胞中表现出最优的激活构象识别能力。

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2. **文献名称**：*BAX Expression in Colorectal Cancer: Prognostic Implications*

**作者**：Chen L, et al.

**摘要**：利用免疫组化技术分析BAX蛋白在结直肠癌组织中的表达水平，发现低BAX表达与化疗耐药性显著相关，提示其可作为预后生物标志物。

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3. **文献名称**：*Optimization of BAX Detection in Neuronal Apoptosis Models*

**作者**：Wang X, et al.

**摘要**：通过优化流式细胞术条件，成功提高了BAX抗体在小鼠脑组织凋亡神经元中的检测灵敏度，为神经退行性疾病研究提供了可靠方法。

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如需真实文献，建议通过PubMed或Google Scholar检索关键词如“BAX antibody validation”或“BAX apoptosis application”。

背景信息

The BAX antibody is a crucial tool in apoptosis research, targeting the BCL-2-associated X protein (BAX), a pro-apoptotic member of the Bcl-2 family. BAX plays a pivotal role in mitochondrial-mediated apoptosis by permeabilizing the mitochondrial outer membrane, triggering cytochrome c release and caspase activation. Under normal conditions, BAX resides in the cytosol as an inactive monomer but undergoes conformational activation and oligomerization upon apoptotic stimuli, such as DNA damage or cellular stress. Antibodies against BAX are widely used to detect its expression, localization, and activation status in cells and tissues. They enable applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry, aiding studies in cancer, neurodegeneration, and developmental biology. Researchers often validate BAX antibodies using knockout cell lines or tissues to ensure specificity, as cross-reactivity with other Bcl-2 family proteins (e.g., BAK) can occur. Both monoclonal and polyclonal formats are available, with epitopes targeting distinct regions (e.g., N-terminal, C-terminal, or active conformation-specific domains). BAX dysregulation is linked to diseases, including chemotherapy resistance in tumors or excessive cell death in neurodegenerative disorders, making these antibodies vital for mechanistic and therapeutic investigations. Proper validation and optimization are critical due to variable performance across experimental conditions.

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