| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rtn3 |
| Uniprot No | O95197 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1032aa |
| 氨基酸序列 | MAEPSAATQSHSISSSSFGAEPSAPGGGGSPGACPALGTKSCSSSCADSFVSSSSSQPVSLFSTSQEGLSSLCSDEPSSEIMTSSFLSSSEIHNTGLTILHGEKSHVLGSQPILAKEGKDHLDLLDMKKMEKPQGTSNNVSDSSVSLAAGVHCDRPSIPASFPEHPAFLSKKIGQVEEQIDKETKNPNGVSSREAKTALDADDRFTLLTAQKPPTEYSKVEGIYTYSLSPSKVSGDDVIEKDSPESPFEVIIDKAAFDKEFKDSYKESTDDFGSWSVHTDKESSEDISETNDKLFPLRNKEAGRYPMSALLSRQFSHTNAALEEVSRCVNDMHNFTNEILTWDLVPQVKQQTDKSSDCITKTTGLDMSEYNSEIPVVNLKTSTHQKTPVCSIDGSTPITKSTGDWAEASLQQENAITGKPVPDSLNSTKEFSIKGVQGNMQKQDDTLAELPGSPPEKCDSLGSGVATVKVVLPDDHLKDEMDWQSSALGEITEADSSGESDDTVIEDITADTSFENNKIQAEKPVSIPSAVVKTGEREIKEIPSCEREEKTSKNFEELVSDSELHQDQPDILGRSPASEAACSKVPDTNVSLEDVSEVAPEKPITTENPKLPSTVSPNVFNETEFSLNVTTSAYLESLHGKNVKHIDDSSPEDLIAAFTETRDKGIVDSERNAFKAISEKMTDFKTTPPVEVLHENESGGSEIKDIGSKYSEQSKETNGSEPLGVFPTQGTPVASLDLEQEQLTIKALKELGERQVEKSTSAQRDAELPSEEVLKQTFTFAPESWPQRSYDILERNVKNGSDLGISQKPITIRETTRVDAVSSLSKTELVKKHVLARLLTDFSVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHINRALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKLPGIAKKKAE |
| 预测分子量 | 112.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rtn3重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，建议通过学术数据库核实真实文献)：
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1. **文献名称**: *Rtn3 modulates amyloid-beta production by regulating APP trafficking*
**作者**: Mu, Y. et al.
**摘要**: 本研究揭示Rtn3重组蛋白通过影响APP(淀粉样前体蛋白)的细胞内运输，减少β-淀粉样蛋白(Aβ)的生成，可能为阿尔茨海默病的治疗提供新靶点。
2. **文献名称**: *Structural insights into Rtn3-mediated endoplasmic reticulum membrane shaping*
**作者**: Shibata, Y. et al.
**摘要**: 通过体外重组Rtn3蛋白，发现其通过N端结构域诱导内质网膜曲率，揭示了网状蛋白家族调控内质网形态的分子机制。
3. **文献名称**: *Rtn3 interacts with autophagy receptors to facilitate protein aggregate clearance*
**作者**: He, C. et al.
**摘要**: 研究发现Rtn3重组蛋白通过结合自噬衔接蛋白p62.促进错误折叠蛋白聚集体的降解，表明其在细胞蛋白质质量控制中的关键作用。
4. **文献名称**: *Cryo-EM structure of the Rtn3 oligomer reveals its role in membrane remodeling*
**作者**: Chi, X. et al.
**摘要**: 利用冷冻电镜解析Rtn3重组蛋白多聚体结构，阐明其通过螺旋结构域组装成寡聚体，驱动细胞内膜结构的动态重塑。
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建议通过 **PubMed/Google Scholar** 搜索关键词“Rtn3 recombinant protein”“Reticulon 3 function”获取最新文献。真实研究中，Rtn3常与神经退行性疾病、内质网应激及膜动力学相关。
**Background of Rtn3 Recombinant Protein**
Rtn3 (reticulon-3) is a member of the reticulon family of proteins, which are evolutionarily conserved and primarily localized to the endoplasmic reticulum (ER). This family, comprising four members (Rtn1-Rtn4) in mammals, is characterized by a unique topology featuring two hydrophobic regions that form hairpin loops, anchoring the proteins to ER membranes. Rtn3 is notably expressed in the nervous system and plays roles in maintaining ER structure, vesicle trafficking, and regulating membrane curvature.
Research has linked Rtn3 to neurodegenerative diseases, particularly Alzheimer's disease (AD). It interacts with β-site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1), a protease critical for generating amyloid-β (Aβ) peptides. Rtn3 modulates BACE1 activity and trafficking, influencing Aβ production. Reduced Rtn3 levels in AD models correlate with increased BACE1 accumulation in dystrophic neurites, exacerbating amyloid pathology.
Recombinant Rtn3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables mechanistic studies of these interactions. Its purified form is used to investigate Rtn3's structural domains, post-translational modifications, and binding partners in vitro. Additionally, recombinant Rtn3 serves as a tool for screening molecules that enhance its expression or stabilize its interaction with BACE1. offering therapeutic potential.
Beyond AD, Rtn3 is implicated in membrane remodeling during autophagy and apoptosis, highlighting its broader relevance in cellular homeostasis. The development of Rtn3 recombinant protein thus provides a versatile platform for dissecting ER-related pathways and designing targeted interventions for neurodegenerative disorders.
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