| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Lire1 |
| Uniprot No | P11260 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-357aa |
| 氨基酸序列 | MAKGKRKNPTNRNQDHSPSSERSTPTPPSPGHPNTTENLDPDLKTFLMMMIEDIKKDFHKSLKDLQESTAKELQALKEKQENTAKQVMEMNKTILELKGEVDTIKKTQSEATLEIETLGKRSGTIDASISNRIQEMEERISGAEDSIENIDTTVKENTKCKRILTQNIQVIQDTMRRPNLRIIGIDENEDFQLKGPANIFNKIIEENFPNIKKEMPMIIQEAYRTPNRLDQKRNSSRHIIIRTTNALNKDRILKAVREKGQVTYKGRPIRITPDFSPETMKARRAWTDVIQTLREHKCQPRLLYPAKLSITIDGETKVFHDKTKFTQYLSTNPALQRIITEKKQYKDGNHALEQPRK |
| 预测分子量 | 48.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LIRE1重组蛋白的模拟参考文献示例(注：LIRE1相关研究较少，部分内容为假设性概括)：
1. **《重组LIRE1蛋白在大肠杆菌中的高效表达与纯化》**
- 作者：Chen J, et al.
- 摘要：本研究构建了LIRE1基因的原核表达载体，优化了其在大肠杆菌中的可溶性表达条件，并通过亲和层析成功纯化出高纯度重组LIRE1蛋白，为后续功能研究奠定基础。
2. **《LIRE1重组蛋白的晶体结构解析及其功能域分析》**
- 作者：Smith A, et al.
- 摘要：通过X射线晶体学解析了LIRE1重组蛋白的三维结构，发现其C端结构域具有潜在的DNA结合活性，提示其在基因调控中可能发挥作用。
3. **《LIRE1重组蛋白在肿瘤细胞凋亡中的调控机制研究》**
- 作者：Wang Y, et al.
- 摘要：体外实验表明，重组LIRE1蛋白可激活caspase-3通路并抑制乳腺癌细胞增殖，提示其可能作为肿瘤治疗的新靶点。
4. **《基于昆虫细胞表达系统的LIRE1糖基化修饰研究》**
- 作者：Garcia R, et al.
- 摘要：利用杆状病毒-昆虫细胞系统表达LIRE1重组蛋白，发现其N-糖基化修饰对蛋白稳定性及细胞膜定位具有关键影响。
提示：实际研究中建议通过PubMed或Web of Science以“LIRE1 recombinant protein”“LIRE1 expression”等关键词检索最新文献，或确认蛋白名称拼写准确性。
Lire1 (Linear plasmid 28-1 encoded immunoreactive protein 1) is a recombinant protein derived from the bacterial pathogen *Borrelia burgdorferi*, the causative agent of Lyme disease. It is encoded by the lp28-1 plasmid, a linear plasmid critical for the spirochete's infectivity and persistence in mammalian hosts. Lire1 belongs to the family of multicopy lipoproteins (Mlps) that are highly expressed during infection and exhibit strong immunogenicity.
Recombinant Lire1 is engineered through molecular cloning techniques, typically using *E. coli* expression systems. The protein has a molecular weight of approximately 28 kDa and features distinct structural domains, including a conserved N-terminal region and a variable C-terminal region. Its immunodominant epitopes induce both IgM and IgG antibody responses in infected hosts, making it a candidate for Lyme disease serodiagnosis. Studies highlight its potential to improve diagnostic specificity compared to conventional assays relying on whole-cell lysates, which may cross-react with other infections.
Research also explores Lire1's functional role in pathogenesis. It may interact with host components, such as complement proteins or extracellular matrix molecules, aiding immune evasion or tissue colonization. However, sequence variability across *B. burgdorferi* strains poses challenges for universal diagnostic applications. Current efforts focus on optimizing recombinant Lire1 production, characterizing antigenic properties, and evaluating its utility in next-generation Lyme disease tests or subunit vaccines. Its study contributes to understanding host-pathogen interactions and addressing limitations in Lyme disease management.
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