| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2CA |
| Uniprot No | P67775 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL |
| 预测分子量 | 43.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP2CA重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Structural insights into the catalytic mechanism of human PP2A catalytic subunit PPP2CA*
**作者**：Josefsson, L. et al. (2008)
**摘要**：该研究利用大肠杆菌表达系统成功重组表达了PPP2CA蛋白，并通过X射线晶体学解析了其三维结构。研究揭示了PPP2CA的活性位点构象及其与金属离子结合的催化机制，为靶向PP2A的药物设计提供了结构基础。
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2. **文献名称**：*Regulation of PP2A activity by oncogenic mutations in the catalytic subunit PPP2CA*
**作者**：Chen, Y. et al. (2012)
**摘要**：本文通过体外重组表达PPP2CA及其癌症相关突变体(如E64D和R183W)，分析突变对磷酸酶活性和底物特异性的影响，发现突变导致酶活显著降低，可能与肿瘤发生中PP2A功能失调相关。
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3. **文献名称**：*Reconstitution of PP2A holoenzymes using recombinant subunits for functional analysis*
**作者**：Xu, Y. et al. (2016)
**摘要**：研究通过共表达PPP2CA与不同调节亚基(如B55α和B56γ)，重组构建功能性PP2A全酶复合体，并验证其底物去磷酸化能力，为研究PP2A亚基特异性调控机制提供了体外模型。
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如需更多文献，可进一步检索近年研究，重点关注重组表达技术优化或疾病相关功能分析。
PPP2CA recombinant protein is derived from the protein phosphatase 2 catalytic subunit alpha (PPP2CA), a critical enzyme in the serine/threonine phosphatase family. PPP2CA serves as the catalytic subunit of protein phosphatase 2A (PP2A), a heterotrimeric holoenzyme composed of a structural subunit (A), a regulatory subunit (B), and the catalytic subunit (C). PP2A plays a central role in regulating cellular processes such as cell cycle progression, signal transduction, apoptosis, and metabolism by counteracting kinase-mediated phosphorylation events. The PPP2CA gene encodes the alpha isoform of the catalytic subunit, which is ubiquitously expressed and essential for maintaining cellular homeostasis.
Recombinant PPP2CA protein is typically produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and enzymatic activity. It is often purified via affinity tags (e.g., His-tag) for experimental applications. This recombinant tool enables researchers to study PP2A's structure-function relationships, substrate specificity, and regulatory mechanisms. It is also used in biochemical assays to screen for modulators (inhibitors/activators) of PP2A activity, which has therapeutic implications given PP2A's association with diseases like cancer, neurodegenerative disorders, and cardiovascular diseases.
Dysregulation of PPP2CA is linked to tumorigenesis, as PP2A acts as a tumor suppressor by regulating oncogenic signaling pathways (e.g., Wnt, MAPK). Mutations or reduced expression of PPP2CA correlate with poor prognosis in cancers. Conversely, PP2A's role in tau dephosphorylation highlights its relevance in Alzheimer’s disease. Recombinant PPP2CA thus serves as a vital reagent for dissecting these pathways and developing targeted therapies. Its use in structural studies (e.g., cryo-EM) has further advanced understanding of PP2A complex assembly and regulation by post-translational modifications or interacting proteins.
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