| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP5C |
| Uniprot No | P53041 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-499aa |
| 氨基酸序列 | AMAEGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECNKIVKQKAFERAIAGDEHKRSVVDSLDIESMTIEDEYSGPKLEDGKVTISFMKELMQWYKDQKKLHRKCAYQILVQVKEVLSKLSTLVETTLKETEKITVCGDTHGQFYDLLNIFELNGLPSETNPYIFNGDFVDRGSFSVEVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWLPLAQCINGKVLIMHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSDPQPQNGRSISKRGVSCQFGPDVTKAFLEENNLDYIIRSHEVKAEGYEVAHGGRCVTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYANTLLQLGMM |
| 预测分子量 | 64.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP5C重组蛋白的3篇代表性文献概览(注：以下内容基于学术领域常见研究方向构建，供参考)：
1. **标题**：*"Recombinant expression and functional characterization of protein phosphatase 5 (PPP5C) in DNA damage response"*
**作者**：Smith A.B., et al.
**摘要**：该研究通过大肠杆菌系统成功表达并纯化重组PPP5C蛋白，证实其磷酸酶活性依赖于TPR结构域与HSP90的相互作用。体外实验显示，PPP5C通过去磷酸化ATM/ATR下游靶点参与DNA损伤修复的负调控。
2. **标题**：*"Structural insights into the autoinhibition and HSP90-mediated activation of PPP5C"*
**作者**：Chen L., Wang Y.
**摘要**：利用重组PPP5C蛋白进行X射线晶体学分析，揭示了其TPR结构域通过分子内相互作用抑制催化活性的自抑制机制。研究进一步证明，HSP90的结合通过解除这种抑制增强PPP5C对MAP激酶的磷酸酶活性，为靶向PPP5C-HSP90复合物的药物设计提供结构基础。
3. **标题**：*"PPP5C regulates tau phosphorylation via CDK5 signaling in Alzheimer’s disease models"*
**作者**：Kim J.H., et al.
**摘要**：通过表达重组PPP5C蛋白的体外磷酸化实验，发现其直接去磷酸化tau蛋白的Ser396位点。在神经元模型中，PPP5C过表达显著降低Aβ诱导的tau过度磷酸化，提示其在阿尔茨海默病中通过调控CDK5/p25通路发挥神经保护作用。
(注：若需真实文献，建议在PubMed或SciFinder中以“PPP5C recombinant”或“Protein phosphatase 5 expression”为关键词检索。)
**Background of PPP5C Recombinant Protein**
PPP5C, also known as protein phosphatase 5 catalytic subunit, is a serine/threonine phosphatase belonging to the PPP (phosphoprotein phosphatase) family. It plays a critical role in cellular signaling by regulating dephosphorylation events involved in stress responses, cell cycle progression, and apoptosis. Structurally, PPP5C contains a catalytic domain and a unique N-terminal tetratricopeptide repeat (TPR) domain, which mediates interactions with heat shock proteins (e.g., HSP90) and other chaperones, modulating its activity and substrate specificity.
Recombinant PPP5C protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. This recombinant form retains enzymatic activity and is widely used to study PPP5C’s biochemical properties, substrate interactions, and regulatory mechanisms in vitro. Its applications span enzyme kinetics assays, structural studies, and screening for inhibitors or activators.
Research highlights PPP5C’s involvement in diverse pathways, including glucocorticoid receptor signaling, DNA damage repair, and tau phosphorylation in neurodegenerative diseases like Alzheimer’s. Dysregulation of PPP5C has been linked to cancer, metabolic disorders, and neurodegeneration, making it a potential therapeutic target. The availability of recombinant PPP5C facilitates mechanistic studies, aiding drug discovery and functional genomics. Efforts to characterize its interactions with co-regulators (e.g., HSP90) and substrates continue to uncover its context-dependent roles in health and disease.
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