| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | bioB |
| Uniprot No | Q5LEY1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-748aa |
| 氨基酸序列 | MTIEEIKNQVLQGTAISREQAEWLALYPRKEELYDAAHDITTACASQEFDMCSIINARSGRCPENCKWCAQSSHYKTKADVYDLVSADECLRQAKYNEAQGVNRFSLVTSGRKPSPKNMKELCVAARRMRRHSSIRLCASLGLLDEEELQALYDAGVTRYHCNLETAPSHFDSLCTTHTQEQKLKTLHAARRVGMDLCCGGIIGMGETVEQRIEFAFTLRDLNIQSIPINLLQPIPGTPLEHQSPLSEEEILTTVALFRFINPAAYLRFAGGRSQLTPEAVRKSLYIGINSAIVGDLLTTLGSKVSDDKEMILSEGYHFADSQFDREHLWHPYTSTSNPLPVYKVKRADGATITLESGQTLIEGMSSWWCAVHGYNHPILNQAVQDQLSRMSHVMFGGLTHDPAIELGKLLLPLVPPSMQKIFYADSGSVAVEVALKMAVQYWYAAGKPEKNNFVTIRNGYHGDTWNAMSVCDPVTGMHSIFGSALPIRHFLPAPSSRFGDEWNPEDIRPLEYLLEKHADELAAFILEPIVQGAGGMRFYHPEYLKEAARLCHRYGVLLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKALTGGYMTLSAVLTTNEVADCISNHAPGAFMHGPTFMGNPLACAVACASVRLLLTSGWQENVKRIEAQLNRELAPARELPQVADVRVLGAIGVIEMKEPVNMAYLQRRFVEEGIWLRPFGKLIYVMPPFIITPEQLTKLTEGMIRIISNGLPGSQTK |
| 预测分子量 | 90.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于bioB重组蛋白的3-4篇参考文献示例(部分信息为模拟内容，实际引用时请核实原文)：
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1. **标题**: *Cloning, expression, and purification of biotin synthase (bioB) from Escherichia coli*
**作者**: Sanyal I, Flint DH
**摘要**: 研究报道了大肠杆菌bioB基因的克隆与重组表达，通过亲和层析纯化获得高纯度蛋白，并验证其催化生物素合成的活性，确认其对硫原子插入的依赖性。
2. **标题**: *Crystal structure of biotin synthase (bioB) reveals a flexible active site for radical-mediated sulfur insertion*
**作者**: Berkovitch F, Nicolet Y, et al.
**摘要**: 通过X射线晶体学解析了重组BioB蛋白的三维结构，揭示了活性位点中铁硫簇和S-腺苷甲硫氨酸的结合模式，提出自由基介导的硫转移催化机制。
3. **标题**: *Radical SAM-dependent catalysis of biotin synthase: Spectroscopic evidence for a [2Fe-2S] cluster intermediate*
**作者**: Jameson GN, et al.
**摘要**: 利用光谱学手段分析重组BioB蛋白催化过程，证实其依赖自由基SAM机制，并检测到关键铁硫簇中间体，阐明硫原子来源与活性位点动态变化。
4. **标题**: *Functional characterization of bioB mutants in Arabidopsis thaliana: Implications for biotin biosynthesis*
**作者**: Li Y, Wang X
**摘要**: 通过拟南芥中重组BioB蛋白的突变体分析，揭示特定半胱氨酸残基对酶活性的必要性，为植物生物素合成途径的工程改造提供依据。
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**注意**：以上文献信息为示例性质，实际引用时请以真实论文数据为准(可通过PubMed、Web of Science等平台检索关键词“bioB recombinant protein”或“biotin synthase expression”获取原文)。
**Background of BioB Recombinant Protein**
BioB recombinant protein refers to a genetically engineered form of the BioB enzyme, derived from the *bioB* gene, which plays a critical role in biotin (vitamin B7) biosynthesis. Biotin is an essential cofactor involved in carboxylation reactions across all domains of life. In bacteria, the *bio* operon (*bioF, bioA, bioD, bioB*) encodes enzymes responsible for converting precursor molecules like pimeloyl-CoA into biotin. Among these, BioB (biotin synthase) catalyzes the final step: the insertion of a sulfur atom into dethiobiotin (DTB) to form biotin. This reaction requires iron-sulfur (Fe-S) clusters and S-adenosylmethionine (SAM) as cofactors, making BioB a member of the radical SAM enzyme superfamily.
Recombinant BioB is typically produced by cloning the *bioB* gene into expression vectors (e.g., *E. coli* or yeast systems) to enable large-scale protein production. Its study is crucial for understanding Fe-S cluster-dependent catalysis, radical-based enzymatic mechanisms, and biotin biosynthesis regulation. Additionally, BioB has applications in industrial biotechnology, such as optimizing biotin production in microbial cell factories.
Challenges in BioB recombinant protein production include maintaining Fe-S cluster integrity during purification and ensuring proper enzymatic activity. Structural studies using recombinant BioB have provided insights into its substrate-binding pockets and catalytic loops, aiding in enzyme engineering. Furthermore, BioB is explored as a potential antimicrobial target, as humans lack the biotin biosynthesis pathway and rely on dietary intake.
In summary, BioB recombinant protein serves as a vital tool for both fundamental research and biotechnological applications, bridging gaps in metabolic engineering, enzymology, and drug development.
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