| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF207 |
| Uniprot No | Q6ZRF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-634aa |
| 氨基酸序列 | MSGAIFGPLEGPSSLDAPSIHPLVCPLCHVQYERPCLLDCFHDFCAGCLRGRATDGRLTCPLCQHQTVLKGPSGLPPVDRLLQFLVDSSGDGVEAVRCANCDLECSEQDVETTYFCNTCGQPLCARCRDETHRARMFARHDIVALGQRSRDVPQKCTLHAEPYLLFSTDKKLLLCIRCFRDMQKESRAHCVDLESAYVQGCERLEQAVLAVKALQTATREAIALLQAMVEEVRHSAAEEEDAIHALFGSMQDRLAERKALLLQAVQSQYEEKDKAFKEQLSHLATLLPTLQVHLVICSSFLSLANKAEFLDLGYELMERLQGIVTRPHHLRPIQSSKIASDHRAEFARCLEPLLLLGPRRVAAAASGANTLAGGLGPKALTGPHCPSPVGKMSGSPVQKPTLHRSISTKVLLAEGENTPFAEHCRHYEDSYRHLQAEMQSLKDQVQELHRDLTKHHSLIKAEIMGDVLHKSLQLDVQIASEHASLEGMRVVFQEIWEEAYQRVANEQEIYEAQLHDLLQLRQENAYLTTITKQITPYVRSIAKVKERLEPRFQAPVDEQSESLQNTHDDSRNNAASARNNPGSVPEKREKTSEPKGNSWAPNGLSEEPLLKNMDHHRSKQKNGGDVPTWREHPT |
| 预测分子量 | 78.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNF207重组蛋白的3篇示例参考文献(注：内容为示例性概括，实际文献需根据具体研究查询)：
1. **标题**: *RNF207 regulates cardiac potassium channels via ubiquitination*
**作者**: Zhang Y, et al.
**摘要**: 研究揭示了RNF207作为E3泛素连接酶，通过泛素化修饰心脏钾离子通道蛋白(如KCNQ1)，调控心肌细胞复极过程，其功能异常可能导致长QT综合征。
2. **标题**: *Crystal structure of the RNF207 RING domain and its interaction with E2 ubiquitin-conjugating enzymes*
**作者**: Li X, et al.
**摘要**: 通过X射线晶体学解析RNF207的RING结构域三维结构，阐明其与泛素结合酶(如UBE2D)的相互作用机制，为靶向药物设计提供结构基础。
3. **标题**: *RNF207 mutations impair protein stability and function in arrhythmogenic cardiomyopathy*
**作者**: Wang H, et al.
**摘要**: 发现RNF207基因突变导致重组蛋白稳定性下降，削弱其泛素连接酶活性，与致心律失常性心肌病的病理机制相关。
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如需真实文献，建议在PubMed或Web of Science中检索关键词“RNF207 recombinant”或“RNF207 ubiquitin ligase”获取最新研究。
**Background of RNF207 Recombinant Protein**
RNF207 (Ring Finger Protein 207) is a ubiquitously expressed E3 ubiquitin ligase encoded by the *RNF207* gene, playing a critical role in protein ubiquitination and subsequent degradation via the ubiquitin-proteasome system. It contains a conserved RING (Really Interesting New Gene) domain, characteristic of E3 ligases, which facilitates substrate recognition and interaction with E2 ubiquitin-conjugating enzymes. RNF207 has garnered attention for its involvement in cardiac physiology, particularly in regulating ion channel stability and action potential dynamics. Studies suggest it interacts with cardiac potassium channels (e.g., KCNH2/HERG) and sodium channels, influencing electrical signaling and arrhythmia susceptibility.
Recombinant RNF207 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling high-purity production for functional studies. Its recombinant form retains enzymatic activity, allowing researchers to dissect its ubiquitination mechanisms, substrate specificity, and regulatory roles in disease contexts. Beyond cardiology, RNF207 has been linked to metabolic disorders, such as obesity and diabetes, where aberrant ubiquitination pathways contribute to pathogenesis.
Research applications include in vitro binding assays, structural analysis, and screening for modulators to treat arrhythmias or metabolic syndromes. Additionally, RNF207’s potential as a biomarker or therapeutic target underscores its biomedical relevance. Despite progress, its full interactome and tissue-specific functions remain under investigation, highlighting the importance of recombinant tools in advancing mechanistic insights. Overall, RNF207 recombinant protein serves as a vital resource for exploring ubiquitin-mediated proteostasis and its implications in human health.
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