| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | esxT |
| Uniprot No | A0A1N0NKG2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-96aa |
| 氨基酸序列 | MSQITYNHGEIDALVADVKGIINKFQAGLEELQHDIQPLVQQAEGQEATAYQEYQKAWHQSAEDLNQILTQLNAKVDQGNQDAQHTDQSAAGAWHH |
| 预测分子量 | 18.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于esxT重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际引用需核实)：
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1. **文献名称**: *Structural insights into the EsxT protein of the ESX-5 secretion system in Mycobacterium tuberculosis*
**作者**: Smith A, et al.
**摘要**: 本研究解析了结核分枝杆菌ESX-5分泌系统中EsxT蛋白的晶体结构，揭示了其与伴侣蛋白EspG5的相互作用机制，为理解ESX-5底物分泌的分子基础提供了依据。
2. **文献名称**: *Recombinant EsxT protein induces Th1 immune response in murine models*
**作者**: Li Y, et al.
**摘要**: 通过大肠杆菌表达系统成功纯化重组EsxT蛋白，实验证明其可激活小鼠巨噬细胞并诱导Th1型细胞因子分泌，提示其在结核病疫苗开发中的潜在应用。
3. **文献名称**: *Functional characterization of EsxT-EsxS heterodimer in mycobacterial virulence*
**作者**: Chen X, et al.
**摘要**: 研究利用重组EsxT-EsxS异源二聚体，证实其在细菌膜穿孔及宿主细胞损伤中的关键作用，并通过基因敲除实验验证了其对结核分枝杆菌致病力的影响。
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**注**：以上文献为示例性内容，实际研究中请通过PubMed、Google Scholar等平台以关键词“EsxT recombinant protein”“Mycobacterium tuberculosis ESX”检索真实文献。
**Background of esxT Recombinant Protein**
The esxT gene is a component of the *Mycobacterium tuberculosis* (Mtb) ESX-1 secretion system, a specialized type VII secretion system critical for bacterial pathogenesis. Mtb utilizes ESX systems to secrete virulence-associated proteins that manipulate host immune responses, enabling intracellular survival and disease progression. The ESX-1 system, in particular, is essential for phagosomal rupture and bacterial escape into the host cytosol, a key step in Mtb’s ability to evade immune detection and establish infection.
EsxT is a small, secreted protein encoded within the ESX-1 locus, alongside other co-regulated genes like *esxA* (ESAT-6) and *esxB* (CFP-10). Structurally, EsxT shares homology with EsxH and EsxG, featuring a conserved WXG motif characteristic of ESX family proteins. It is believed to function as a chaperone or adaptor, facilitating the secretion or stability of other ESX-1 substrates. Studies suggest EsxT interacts with host cell components, potentially modulating autophagy, membrane dynamics, or inflammatory signaling pathways.
Recombinant EsxT protein is produced through heterologous expression in systems like *E. coli*, enabling biochemical and functional studies. Its purification allows researchers to investigate protein-protein interactions, host-pathogen interfaces, and immune recognition mechanisms. EsxT has also been explored as a candidate for diagnostic tools or subunit vaccines, given its immunogenicity and role in virulence. However, its exact mechanistic contributions to Mtb pathogenesis remain under investigation, with ongoing research aiming to clarify its interplay with other ESX-1 effectors and host targets.
Understanding EsxT’s function provides insights into Mtb’s evasion strategies and supports the development of novel therapeutics targeting ESX-1-dependent pathways, a critical frontier in combating tuberculosis.
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