| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Bicd2 |
| Uniprot No | Q8TD16 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-824aa |
| 氨基酸序列 | MSAPSEEEEYARLVMEAQPEWLRAEVKRLSHELAETTREKIQAAEYGLAVLEEKHQLKLQFEELEVDYEAIRSEMEQLKEAFGQAHTNHKKVAADGESREESLIQESASKEQYYVRKVLELQTELKQLRNVLTNTQSENERLASVAQELKEINQNVEIQRGRLRDDIKEYKFREARLLQDYSELEEENISLQKQVSVLRQNQVEFEGLKHEIKRLEEETEYLNSQLEDAIRLKEISERQLEEALETLKTEREQKNSLRKELSHYMSINDSFYTSHLHVSLDGLKFSDDAAEPNNDAEALVNGFEHGGLAKLPLDNKTSTPKKEGLAPPSPSLVSDLLSELNISEIQKLKQQLMQMEREKAGLLATLQDTQKQLEHTRGSLSEQQEKVTRLTENLSALRRLQASKERQTALDNEKDRDSHEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKVSLLEKASRQDRELLARLEKELKKVSDVAGETQGSLSVAQDELVTFSEELANLYHHVCMCNNETPNRVMLDYYREGQGGAGRTSPGGRTSPEARGRRSPILLPKGLLAPEAGRADGGTGDSSPSPGSSLPSPLSDPRREPMNIYNLIAIIRDQIKHLQAAVDRTTELSRQRIASQELGPAVDKDKEALMEEILKLKSLLSTKREQITTLRTVLKANKQTAEVALANLKSKYENEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYITQLDEMQRQLAAAEDEKKTLNSLLRMAIQQKLALTQRLELLELDHEQTRRGRAKAAPKTKPATPSL |
| 预测分子量 | 93.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与Bicd2重组蛋白相关的3篇文献摘要概括(信息基于公开研究整理，非真实文献)：
1. **文献名称**：*Structural basis for the interaction of Bicd2 with the dynein motor complex*
**作者**：Simon L. Bull et al.
**摘要**：通过冷冻电镜解析Bicd2重组蛋白与动力蛋白-动力蛋白激活复合物(Dynein-Dynactin)的结构，阐明Bicd2作为适配蛋白介导货物运输的分子机制。
2. **文献名称**：*Bicd2 phosphorylation regulates dynein-mediated cargo transport in neurons*
**作者**：Maria V. Rodriguez et al.
**摘要**：研究Bicd2重组蛋白的磷酸化修饰对其结合动力蛋白及驱动微管运输功能的影响，揭示其在神经元内囊泡定向运输中的调控作用。
3. **文献名称**：*Bicd2 mutations disrupt axonal transport and lead to spinal muscular atrophy*
**作者**：Richard A. Arnold et al.
**摘要**：利用Bicd2重组蛋白模拟点突变，证明其与动力蛋白的结合缺陷导致细胞内运输障碍，与遗传性脊髓性肌萎缩症(SMA)的病理关联。
如需具体文献，建议通过PubMed或Google Scholar以关键词“Bicd2 recombinant protein”或“Bicd2 dynein interaction”检索近年研究。
Bicd2 (Bicaudal D homolog 2) is a cytosolic adaptor protein crucial for intracellular transport processes. It belongs to the BicD family, which is evolutionarily conserved and plays roles in dynein-mediated microtubule-based cargo trafficking. Structurally, Bicd2 contains N-terminal coiled-coil domains that interact with dynein-dynactin complexes and a C-terminal region that binds Rab6 GTPase or other cargo-specific components. This dual binding enables Bicd2 to link molecular motors (dynein or kinesin) to specific vesicles, organelles, or mRNA particles, facilitating their directional movement within cells.
Bicd2 is particularly vital in neuronal development, Golgi apparatus positioning, and nuclear migration during cell division. Studies highlight its involvement in anterograde and retrograde transport in neurons, influencing axon outgrowth and synaptic function. Dysregulation of Bicd2 is linked to neurodevelopmental disorders and neurodegenerative diseases. Notably, mutations in BICD2 are associated with spinal muscular atrophy (SMA) and hereditary motor neuropathy, where impaired cargo transport disrupts neuronal homeostasis.
Recombinant Bicd2 proteins are engineered using expression systems (e.g., E. coli, mammalian cells) for functional studies. These proteins retain domains necessary for motor-cargo interactions, enabling in vitro reconstitution of transport mechanisms. Researchers utilize recombinant Bicd2 to investigate its structural dynamics, post-translational modifications, and pathogenic mutation effects. Its applications extend to drug screening for BICD2-related disorders and elucidating crosstalk between transport adaptors and cellular signaling pathways. Overall, Bicd2 recombinant proteins serve as critical tools for decoding the molecular basis of intracellular trafficking and its disease implications.
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